Enhanced separation of filamentous fungi by ultrasonic field: possible usage in repeated batch processes
Usage of ultrasonic field-based filters in retention of filamentous fungal cells was assessed using Rhizopus arrhizus NRRL 1526 as a model organism. Effects of operating conditions, such as power input, harvest pump flow rate, run time and stop time, on the system's separation efficiency (SE) w...
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| Veröffentlicht in: | Journal of biotechnology 2002-08, Vol.97 (2), p.191-197 |
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| creator | Crognale, Silvia Federici, Federico Petruccioli, Maurizio |
| description | Usage of ultrasonic field-based filters in retention of filamentous fungal cells was assessed using
Rhizopus arrhizus NRRL 1526 as a model organism. Effects of operating conditions, such as power input, harvest pump flow rate, run time and stop time, on the system's separation efficiency (SE) were evaluated by modulating the variables according to a Central Composite Design (CCD). The standard pump with which the ultrasonic filter was equipped was shown to be unsuitable and was, therefore, substituted for with a prime rate reverse pump that made possible separation and recycle of the mycelial biomass. The operating conditions were optimised (run time, 300 s; stop time, 3 s; power input, 6 W; harvest pump flow rate, 4 l per day) and a repeated batch process (three batches for a total of 192 h) was performed during which the SE was maintained always higher than 88%. |
| doi_str_mv | 10.1016/S0168-1656(02)00062-7 |
| format | Article |
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Rhizopus arrhizus NRRL 1526 as a model organism. Effects of operating conditions, such as power input, harvest pump flow rate, run time and stop time, on the system's separation efficiency (SE) were evaluated by modulating the variables according to a Central Composite Design (CCD). The standard pump with which the ultrasonic filter was equipped was shown to be unsuitable and was, therefore, substituted for with a prime rate reverse pump that made possible separation and recycle of the mycelial biomass. The operating conditions were optimised (run time, 300 s; stop time, 3 s; power input, 6 W; harvest pump flow rate, 4 l per day) and a repeated batch process (three batches for a total of 192 h) was performed during which the SE was maintained always higher than 88%.</description><subject>Biological and medical sciences</subject><subject>Bioreactors</subject><subject>Biotechnology</subject><subject>Cell separation</subject><subject>Cell Survival</subject><subject>Feasibility Studies</subject><subject>Filtration - instrumentation</subject><subject>Filtration - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungi - chemistry</subject><subject>Methods. Procedures. 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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Biological and medical sciences Bioreactors Biotechnology Cell separation Cell Survival Feasibility Studies Filtration - instrumentation Filtration - methods Fundamental and applied biological sciences. Psychology Fungi - chemistry Methods. Procedures. Technologies Models, Statistical Mycology - instrumentation Mycology - methods Others Perfusion system Pilot Projects Piperazines Repeated batch process Rheology Rhizopus - chemistry Rhizopus - genetics Rhizopus - isolation & purification Rhizopus arrhizus Statistics as Topic Time Factors Ultrasonic filter Ultrasonics Various methods and equipments |
| title | Enhanced separation of filamentous fungi by ultrasonic field: possible usage in repeated batch processes |
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