Forward Translocation Is the Natural Pathway of RNA Release at an Intrinsic Terminator
Intrinsic terminators of bacterial RNA polymerase are small (< ∼30 bp) sequences containing a dyad symmetry that encodes a hairpin in the RNA, followed immediately by a uridine-rich stretch of 5–9 nucleotides just before the site of RNA release. Formation of the RNA hairpin destabilizes the elong...
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| Veröffentlicht in: | Molecular cell 2004-04, Vol.14 (1), p.117-126 |
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| creator | Santangelo, Thomas J Roberts, Jeffrey W |
| description | Intrinsic terminators of bacterial RNA polymerase are small (< ∼30 bp) sequences containing a dyad symmetry that encodes a hairpin in the RNA, followed immediately by a uridine-rich stretch of 5–9 nucleotides just before the site of RNA release. Formation of the RNA hairpin destabilizes the elongation complex, leading to transcript release. We test a model in which hair-pin formation drives RNA polymerase and the melted DNA bubble downstream without transcript elongation, thus releasing the transcript from its enclosure within the enzyme as an RNA/DNA hybrid. We show that blocking downstream translocation of RNAP and preventing downstream DNA unwinding both inhibit transcript release. We argue that translocation of RNA polymerase is essential and that translocation of the bubble stimulates, but is not required, for RNA release; we conclude that forward translocation is the natural pathway of RNA release at an intrinsic terminator. |
| doi_str_mv | 10.1016/S1097-2765(04)00154-6 |
| format | Article |
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Formation of the RNA hairpin destabilizes the elongation complex, leading to transcript release. We test a model in which hair-pin formation drives RNA polymerase and the melted DNA bubble downstream without transcript elongation, thus releasing the transcript from its enclosure within the enzyme as an RNA/DNA hybrid. We show that blocking downstream translocation of RNAP and preventing downstream DNA unwinding both inhibit transcript release. 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| ispartof | Molecular cell, 2004-04, Vol.14 (1), p.117-126 |
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| source | MEDLINE; Cell Press Free Archives; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Free Full-Text Journals in Chemistry |
| subjects | Bacterial Proteins - metabolism Base Sequence DNA - chemistry DNA - metabolism DNA-Directed RNA Polymerases - metabolism Molecular Sequence Data Nucleic Acid Conformation RNA, Bacterial - chemistry RNA, Bacterial - metabolism Terminator Regions, Genetic Transcription, Genetic |
| title | Forward Translocation Is the Natural Pathway of RNA Release at an Intrinsic Terminator |
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