Light-Directed Simultaneous Synthesis of Oligopeptides on Microarray Substrate Using a Photogenerated Acid
Photogenerated acid (PGA) was used as the acid to remove the protection group from amino acids or peptide oligomers. Comparative study of the deprotection using a PGA, trisarylsulfonium antimonyhexafluoride (SSb), and trifluoroacetic acid (TFA) was performed on glass microscope slides. The results s...
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Veröffentlicht in: | Biotechnology progress 2002, Vol.18 (3), p.641-646 |
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Sprache: | eng |
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Zusammenfassung: | Photogenerated acid (PGA) was used as the acid to remove the protection group from amino acids or peptide oligomers. Comparative study of the deprotection using a PGA, trisarylsulfonium antimonyhexafluoride (SSb), and trifluoroacetic acid (TFA) was performed on glass microscope slides. The results showed that PGA can replace TFA in the deprotection step of oligopeptide synthesis with comparable efficiencies. Acids needed for the deprotection step were generated in situ by light activiation of the precursor molecule on the microwell substrate. A maskless laser light illumination system was used to activate the precursor. The accuracy of the amino acid sequence of the synthesized oligopeptide and the location of the synthesis was illustrated by the specific recognition binding of two different models: lead(II) ion‐peptide biosensor for lead(II) and human protein p53 (residue 20–25)‐mouse MAb DO1. After parallel synthesis of the target peptide models and their analogues based on the predetermined pattern, specific binding treatment, and fluorescence labeling, the fluorescence emission images of the oligopeptide microarray showed fluorescence intensity as a result of specific binding at the correct locations of the array. The stepwise synthesis efficiencies of pentapeptide synthesis on the microwell substrate range are ∼96–100% and do not decrease with respect to the chain length of the peptide. |
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ISSN: | 8756-7938 1520-6033 |
DOI: | 10.1021/bp015505n |