Inheritance and organisation of the mitochondrial genome differ between two Saccharomyces yeasts
Petite-positive Saccharomyces yeasts can be roughly divided into the sensu stricto, including Saccharomyces cerevisiae, and sensu lato group, including Saccharomyces castellii; the latter was recently studied for transmission and the organisation of its mitochondrial genome. S. castellii mitochondri...
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Veröffentlicht in: | Journal of molecular biology 2002-05, Vol.318 (3), p.627-636 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Petite-positive
Saccharomyces yeasts can be roughly divided into the sensu stricto, including
Saccharomyces cerevisiae, and sensu lato group, including
Saccharomyces castellii; the latter was recently studied for transmission and the organisation of its mitochondrial genome.
S.
castellii
mitochondrial molecules (mtDNA) carrying point mutations, which confer antibiotic resistance, behaved in genetic crosses as the corresponding point mutants of
S.
cerevisiae
. While
S.
castellii
generated spontaneous petite mutants in a similar way as
S.
cerevisiae
, the petites exhibited a different inheritance pattern. In crosses with the wild type strains a majority of
S.
castellii
petites was neutral, and the suppressivity in suppressive petites was never over 50%. The two yeasts also differ in organisation of their mtDNA molecules. The 25,753
bp sequence of
S.
castellii
mtDNA was determined and the coding potential of both yeasts is similar. However, the
S.
castellii
intergenic sequences are much shorter and do not contain sequences homologous to the
S.
cerevisiae
biologically active intergenic sequences, as
ori/
rep/
tra, which are responsible for the hyper-suppressive petite phenotype found in
S.
cerevisiae
. The structure of one suppressive
S.
castellii
mutant, CA38, was also determined. Apparently, a short direct intergenic repeat was involved in the generation of this petite mtDNA molecule. |
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ISSN: | 0022-2836 1089-8638 |
DOI: | 10.1016/S0022-2836(02)00037-2 |