Functional characterization of the human atrial essential myosin light chain (hALC-1) in a transgenic rat model

Most patients with hypertrophic cardiomyopathy and congenital heart diseases express the atrial essential myosin light chains (ALC-1) in their ventricles, partially replacing the ventricular essential light chains (VLC-1). This VLC-1/ALC-1 isoform shift is correlated with an increase in cross-bridge...

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Veröffentlicht in:Journal of molecular medicine (Berlin, Germany) Germany), 2004-04, Vol.82 (4), p.265-274
Hauptverfasser: AHMED IHAB ABDELAZIZ, SEGARIC, Jadranka, MORANO, Ingo, BARTSCH, Holger, PETZHOLD, Daria, SCHLEGEL, Wolfgang-Peter, KOTT, Monika, SEEFELDT, Ingo, KLOSE, Joachim, BADER, Michael, HAASE, Hannelore
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Sprache:eng
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Zusammenfassung:Most patients with hypertrophic cardiomyopathy and congenital heart diseases express the atrial essential myosin light chains (ALC-1) in their ventricles, partially replacing the ventricular essential light chains (VLC-1). This VLC-1/ALC-1 isoform shift is correlated with an increase in cross-bridge cycling kinetics as measured using skinned fibers from the hypertrophied ventricles of human hearts. To study the functional importance of hALC-1 in the intact perfused heart, we generated a transgenic rat model (TGR) overexpressing hALC-1 in the heart. Twelve-week-old TGR rats expressed 17 +/- 4 microg hALC-1 per mg of whole SDS-soluble protein. Their perfused heart contractility parameters were evaluated using the Langendorff preparation. Expression of hALC-1 was accompanied by statistically significant improvements (P
ISSN:0946-2716
1432-1440
DOI:10.1007/s00109-004-0525-4