Complete mapping of a novel HLA A6801-restricted HIV-1 Tat epitope directly with a rapid modified enzyme-linked immunospot assay

We identified a novel HLA A*6801-restricted HIV-1 Tat-derived cytotoxic T lymphocyte (CTL) epitope using an adapted enzyme-linked immunospot assay that allows the rapid ex vivo identification of CTL epitopes together with their associated HLA Class I restriction elements. The optimal 11 amino acid r...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	AIDS (London) 2002-06, Vol.16 (9), p.1285-1287
Hauptverfasser:	Oxenius, Annette, Jakobsen, Bent K, Easterbrook, Philippa J, Boulter, Jonathan M, Tun, Tin, Waters, Anele, Agudelo, Juliet, Barnardo, Martin, Phillips, Rodney E, Price, David A
Format:	Artikel
Sprache:	eng
Schlagworte:	AIDS/HIV Enzyme-Linked Immunosorbent Assay Epitope Mapping - methods Epitopes, T-Lymphocyte - immunology Gene Products, tat - chemistry Gene Products, tat - immunology HIV-1 - immunology HLA-A Antigens - immunology Humans tat Gene Products, Human Immunodeficiency Virus
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	We identified a novel HLA A*6801-restricted HIV-1 Tat-derived cytotoxic T lymphocyte (CTL) epitope using an adapted enzyme-linked immunospot assay that allows the rapid ex vivo identification of CTL epitopes together with their associated HLA Class I restriction elements. The optimal 11 amino acid residue Tat epitope efficiently stabilized the refolding of monomeric peptide-HLA A6801 complexes in vitro and fluorochrome-labelled, tetrameric peptide-HLA A6801 complexes stained CD8 T cells specific for this epitope directly ex vivo.
ISSN:	0269-9370