Multidrug resistance-associated protein 4 is up-regulated in liver but down-regulated in kidney in obstructive cholestasis in the rat

Multidrug resistance-associated protein 4 (Mrp4, ABCC4) transports cyclic nucleotides, anti-retroviral compounds, and sulfated bile acids. Mrp4 expression is increased in farnesyl/bile acid receptor knockout mice. Our aim was to investigate Mrp4 expression and function in rat liver and kidney in obs...

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Veröffentlicht in:	Journal of hepatology 2004-04, Vol.40 (4), p.585-591
Hauptverfasser:	Denk, Gerald U, Soroka, Carol J, Takeyama, Yasuaki, Chen, Wen-Sheng, Schuetz, John D, Boyer, James L
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals ATP-binding cassette transporter Base Sequence Bile acids Bile Acids and Salts - blood Bile Acids and Salts - urine Bile duct ligation Biological and medical sciences Cholestasis - genetics Cholestasis - metabolism Cyclic adenosine monophosphate Cyclic AMP - blood Cyclic AMP - urine Cyclic nucleotides DNA - genetics Down-Regulation Gastroenterology. Liver. Pancreas. Abdomen Immunohistochemistry Kidney - metabolism Liver - metabolism Liver. Biliary tract. Portal circulation. Exocrine pancreas Male Medical sciences Multidrug resistance-associated protein 4 Multidrug Resistance-Associated Proteins - genetics Multidrug Resistance-Associated Proteins - metabolism Other diseases. Semiology Rats Rats, Sprague-Dawley Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - genetics RNA, Messenger - metabolism Up-Regulation
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Zusammenfassung:	Multidrug resistance-associated protein 4 (Mrp4, ABCC4) transports cyclic nucleotides, anti-retroviral compounds, and sulfated bile acids. Mrp4 expression is increased in farnesyl/bile acid receptor knockout mice. Our aim was to investigate Mrp4 expression and function in rat liver and kidney in obstructive cholestasis. Male Sprague–Dawley rats were subjected to bile duct ligation (BDL) or sham-surgery. Animals were sacrificed after 3, 7, and 14 days and tissues were harvested for Western blot analysis, real-time reverse transcriptase-mediated polymerase chain reaction (RT-PCR), and immunohistochemistry. Western blot analysis revealed a progressive, more than seven-fold increase (P
ISSN:	0168-8278 1600-0641
DOI:	10.1016/j.jhep.2003.12.001