A two dimensional electrophoresis database of a human Jurkat T-cell line

About 2000 protein spots of human Jurkat T‐cells were detected by high resolution two‐dimensional gel electrophoresis (2‐DE) and were characterized in terms of their isoelectric point and molecular mass. A 2‐DE database was constructed and is available at http://www.mpiib‐berlin.mpg.de/2D‐PAGE/. At present the database contains 67 identified protein spots. These proteins were identified after tryptic digestion by peptide mass fingerprinting with delayed extraction‐matrix assisted laser desorption/ionization‐mass spectrometry (DE‐MALDI‐MS). Proteins with a sequence coverage of at least 30% were introduced in the database. This sequence coverage could not always be obtained by using only the matrix α‐cyano‐4‐hydroxycinnamic acid (CHCA) for the mass analysis. Therefore, an additional mass spectrum was recorded by using 2,5‐dihydroxybenzoic acid (DHB). Usually, additional mass peaks were detected and together with the mass spectrum of CHCA this resulted in the desired sequence coverage.