Structure of the DNA binding domain of E. coli SSB bound to ssDNA

The structure of the homotetrameric DNA binding domain of the single stranded DNA binding protein from Escherichia coli (Eco SSB) bound to two 35-mer single stranded DNAs was determined to a resolution of 2.8 Å. This structure describes the vast network of interactions that results in the extensive...

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Veröffentlicht in:	Nat. Struct. Biol 2000-08, Vol.7 (8), p.648-652
Hauptverfasser:	Lohman, Timothy M, Waksman, Gabriel, Raghunathan, Srinivasan, Kozlov, Alexander G
Format:	Artikel
Sprache:	eng
Schlagworte:	BASIC BIOLOGICAL SCIENCES Binding Sites Biochemistry Biological Microscopy Biomedical and Life Sciences Crystallography, X-Ray Deoxyribonucleic acid DNA DNA, Single-Stranded - chemistry DNA, Single-Stranded - genetics DNA, Single-Stranded - metabolism DNA-Binding Proteins - chemistry DNA-Binding Proteins - metabolism E coli Escherichia coli Escherichia coli - chemistry letter Life Sciences Membrane Biology Models, Molecular MOLECULAR BIOLOGY Molecular Sequence Data NATIONAL SYNCHROTRON LIGHT SOURCE NSLS Oligodeoxyribonucleotides - chemistry Oligodeoxyribonucleotides - genetics Oligodeoxyribonucleotides - metabolism Peptide Fragments - chemistry Peptide Fragments - metabolism Protein Binding Protein Structure Protein Structure, Quaternary single-stranded DNA-binding protein Spectrometry, Fluorescence Structure-Activity Relationship Thermodynamics
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Zusammenfassung:	The structure of the homotetrameric DNA binding domain of the single stranded DNA binding protein from Escherichia coli (Eco SSB) bound to two 35-mer single stranded DNAs was determined to a resolution of 2.8 Å. This structure describes the vast network of interactions that results in the extensive wrapping of single stranded DNA around the SSB tetramer and suggests a structural basis for its various binding modes.
ISSN:	1072-8368 1545-9993 2331-365X 1545-9985
DOI:	10.1038/77943