Enriched gonadal migration of donor-derived gonadal primordial germ cells by immunomagnetic cell sorting in birds

This study was conducted to evaluate whether immunomagnetic treatment could improve the retrieval and migration capacity of avian gonadal primordial germ cells (gPGCs) collected from gonads in 5.5‐day‐old chick and 5‐day‐old quail embryos, respectively. Collected gPGCs were loaded into a magnetic‐ac...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Molecular reproduction and development 2004-05, Vol.68 (1), p.81-87
Hauptverfasser:	Kim, Jin Nam, Kim, Mi A, Park, Tae Sub, Kim, Duk Kyung, Park, Hyun Jeong, Ono, Tamao, Lim, Jeong Mook, Han, Jae Yong
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Birds Cell Count Cell Movement chicken Chickens Early stages. Segmentation. Gastrulation. Neurulation Embryology: invertebrates and vertebrates. Teratology Fundamental and applied biological sciences. Psychology Germ Cells - cytology Germ Cells - immunology gonadal migration gonadal primordial germ cell (gPGC) Gonads - cytology Gonads - immunology Immunomagnetic Separation - instrumentation Immunomagnetic Separation - methods immunomagnetic treatment Quail
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	This study was conducted to evaluate whether immunomagnetic treatment could improve the retrieval and migration capacity of avian gonadal primordial germ cells (gPGCs) collected from gonads in 5.5‐day‐old chick and 5‐day‐old quail embryos, respectively. Collected gPGCs were loaded into a magnetic‐activated cell sorter (MACS) after being conjugated with specific gPGC antibodies and either MACS‐treated or non‐treated cells in each species were subsequently transferred to the recipient embryos. MACS treatment significantly (P
ISSN:	1040-452X 1098-2795
DOI:	10.1002/mrd.20051