3D reconstruction of the hemocyanin subunit dimer from the chiton Acanthochiton fascicularis

3D reconstruction of the hemocyanin subunit dimer from the chiton Acanthochiton fascicularis

Procedures are presented for the purification of the subunit dimer from Acanthochiton fasicularis hemocyanin. Electron microscopy of negatively stained specimens revealed a uniform population of macromolecules possessing the characteristic “boat shape”. A 3D reconstruction from this EM data generate...

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Veröffentlicht in:Micron (Oxford, England : 1993) England : 1993), 2004, Vol.35 (1), p.23-26
Hauptverfasser: Harris, J.Robin, Meissner, Ulrich, Gebauer, Wolfgang, Markl, Jürgen
Format: Artikel
Sprache:eng
Schlagworte:
3D reconstruction
Animals
Dimerization
Electron microscopy
Gastropod
Hemocyanin
Hemocyanins - chemistry
Microscopy, Electron
Mollusca - chemistry
Negative staining
Protein Subunits
Subunit dimer
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Zusammenfassung:Procedures are presented for the purification of the subunit dimer from Acanthochiton fasicularis hemocyanin. Electron microscopy of negatively stained specimens revealed a uniform population of macromolecules possessing the characteristic “boat shape”. A 3D reconstruction from this EM data generated a ∼3 nm resolution model that correlates well with earlier data of the purported subunit dimer, extracted from the 3D reconstruction of the didecamer of Haliotis tuberculata hemocyanin type 1.
ISSN:0968-4328
1878-4291
DOI:10.1016/j.micron.2003.10.008