Characterization of a Rab7-like GTPase, EhRab7: a marker for the early stages of endocytosis in Entamoeba histolytica

We have identified a 667 base pair Rab7-like cDNA ( EhRab7) from Entamoeba histolytica. The EhRab7 cDNA predicts a polypeptide of at least 206 amino acids with a molecular mass of at least 24.5 kDa. Alignment of EhRab7 with other Rab proteins demonstrated that EhRab7 shared significant homology at t...

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Veröffentlicht in:	Molecular and biochemical parasitology 2002-05, Vol.121 (2), p.254-264
Hauptverfasser:	Welter, Brenda H., Laughlin, Richard C., Temesvari, Lesly A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Base Sequence Biomarkers DNA, Complementary Endocytosis Endosomes - enzymology Entamoeba histolytica Entamoeba histolytica - enzymology Entamoeba histolytica - genetics Entamoeba histolytica - growth & development GTP Phosphohydrolases - chemistry GTP Phosphohydrolases - genetics GTP Phosphohydrolases - metabolism Microscopy, Fluorescence Molecular Sequence Data Phagocytosis Protozoan Proteins - chemistry Protozoan Proteins - genetics Protozoan Proteins - metabolism rab GTP-Binding Proteins - chemistry rab GTP-Binding Proteins - genetics rab GTP-Binding Proteins - metabolism Rab GTPase Secretion Sequence Alignment Sequence Analysis, DNA Sequence Homology, Amino Acid
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Zusammenfassung:	We have identified a 667 base pair Rab7-like cDNA ( EhRab7) from Entamoeba histolytica. The EhRab7 cDNA predicts a polypeptide of at least 206 amino acids with a molecular mass of at least 24.5 kDa. Alignment of EhRab7 with other Rab proteins demonstrated that EhRab7 shared significant homology at the amino acid level with Rab7-like proteins from a number of other eukaryotes, suggesting that EhRab7 is a Rab7 homolog for E. histolytica. Using immunofluorescence microscopy, EhRab7 was demonstrated to be associated with early fluid-phase endosomes (
ISSN:	0166-6851 1872-9428
DOI:	10.1016/S0166-6851(02)00047-6