Search for serum protein-binding disaccharides and disaccharide-binding serum proteins by affinity capillary electrophoresis

The potential use of affinity capillary electrophoresis in a microscale search for mutually interacting substances in biological fluid is demonstrated. Some disaccharides, especially gentiobiose (Gen), derivatized with 1‐phenyl‐3‐methyl‐5‐pyrazolone, caused peak retardation when electrophoresed in a...

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Veröffentlicht in:Electrophoresis 2004-03, Vol.25 (6), p.876-881
Hauptverfasser: Taga, Atsushi, Yamamoto, Yuka, Maruyama, Rie, Honda, Susumu
Format: Artikel
Sprache:eng
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Zusammenfassung:The potential use of affinity capillary electrophoresis in a microscale search for mutually interacting substances in biological fluid is demonstrated. Some disaccharides, especially gentiobiose (Gen), derivatized with 1‐phenyl‐3‐methyl‐5‐pyrazolone, caused peak retardation when electrophoresed in a neutral running buffer, containing human serum. Gen, the most significantly retarded disaccharide, was converted to its negatively charged bis‐mercaptoethanesulfonate derivative (MerESGen), and a serum sample was analyzed in a neutral buffer containing the derivatized disaccharide. Two peaks, belonging to the β‐globulin fraction, were found to be remarkably retarded in the buffer containing MerES‐Gen in a concentration‐dependent way. These findings prove an interaction between disaccharides and serum proteins.
ISSN:0173-0835
1522-2683
DOI:10.1002/elps.200305765