Search for serum protein-binding disaccharides and disaccharide-binding serum proteins by affinity capillary electrophoresis
The potential use of affinity capillary electrophoresis in a microscale search for mutually interacting substances in biological fluid is demonstrated. Some disaccharides, especially gentiobiose (Gen), derivatized with 1‐phenyl‐3‐methyl‐5‐pyrazolone, caused peak retardation when electrophoresed in a...
Gespeichert in:
Veröffentlicht in: | Electrophoresis 2004-03, Vol.25 (6), p.876-881 |
---|---|
Hauptverfasser: | , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | The potential use of affinity capillary electrophoresis in a microscale search for mutually interacting substances in biological fluid is demonstrated. Some disaccharides, especially gentiobiose (Gen), derivatized with 1‐phenyl‐3‐methyl‐5‐pyrazolone, caused peak retardation when electrophoresed in a neutral running buffer, containing human serum. Gen, the most significantly retarded disaccharide, was converted to its negatively charged bis‐mercaptoethanesulfonate derivative (MerESGen), and a serum sample was analyzed in a neutral buffer containing the derivatized disaccharide. Two peaks, belonging to the β‐globulin fraction, were found to be remarkably retarded in the buffer containing MerES‐Gen in a concentration‐dependent way. These findings prove an interaction between disaccharides and serum proteins. |
---|---|
ISSN: | 0173-0835 1522-2683 |
DOI: | 10.1002/elps.200305765 |