A new method for purification of carbonic anhydrase isozymes by affinity chromatography

A new affinity gel for purification of carbonic anhydrase isozymes was prepared using EUPERGIT C-250L derivatized with p-aminobenzenesulfonamide, an inhibitor of carbonic anhydrase. The binding capacity of the affinity gel was determined at different temperatures, pH values, elution buffers, and ion...

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Veröffentlicht in:	Biochemistry (Moscow) 2004-02, Vol.69 (2), p.216-219
Hauptverfasser:	Ozensoy, O, Arslan, O, Sinan, S Oznur
Format:	Artikel
Sprache:	eng
Schlagworte:	Affinity chromatography Animals Carbonic anhydrase inhibitors Carbonic Anhydrases - isolation & purification Cattle Chromatography, Affinity - methods Humans Hydrogen-Ion Concentration Isoenzymes - isolation & purification Osmolar Concentration Protein folding Resins, Synthetic Sulfonamides - chemistry
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Zusammenfassung:	A new affinity gel for purification of carbonic anhydrase isozymes was prepared using EUPERGIT C-250L derivatized with p-aminobenzenesulfonamide, an inhibitor of carbonic anhydrase. The binding capacity of the affinity gel was determined at different temperatures, pH values, elution buffers, and ionic strengths. Human carbonic anhydrase isozymes (HCA I and HCA II) and bovine carbonic anhydrase (BCA) were purified in high yields from erythrocytes.
ISSN:	0006-2979 1608-3040
DOI:	10.1023/B:BIRY.0000018954.38006.9a