Propeptin, a New Inhibitor of Prolyl Endopeptidase Produced by Microbispora: II. Determination of Chemical Structure

The structure of propeptin, a new inhibitor of prolyl endopeptidase isolated from Microbispora sp. SNA-115, was determined. FAB/MS, Edman degradation and amino acid analysis revealed propeptin to be a cyclic polypeptide consisting of 19 common L-amino acids. By FAB/MS and protein chemical methods, t...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of antibiotics 2002/03/25, Vol.55(3), pp.296-300
Hauptverfasser:	ESUMI, YASUAKI, SUZUKI, YOSHIKATSU, ITOH, YUMIKO, URAMOTO, MASAKAZU, KIMURA, KEN-ICHI, GOTO, MASAAKI, YOSHIHAMA, MAKOTO, ICHIKAWA, TERUO
Format:	Artikel
Sprache:	eng
Schlagworte:	Actinomycetales - metabolism Amino Acid Sequence Anti-Bacterial Agents - biosynthesis Anti-Bacterial Agents - chemistry Anti-Bacterial Agents - pharmacology Antibiotics (antibacterial agents, antifungal agents) Antibiotics, microbial producers, chemotherapic agents, antiseptics, disinfecting agents Antibiotics. Antiinfectious agents. Antiparasitic agents Applied microbiology Biological and medical sciences Fundamental and applied biological sciences. Psychology General aspects Mass Spectrometry - methods Medical sciences Microbiology Molecular Sequence Data Peptides, Cyclic - biosynthesis Peptides, Cyclic - chemistry Peptides, Cyclic - pharmacology Pharmacology. Drug treatments Serine Endopeptidases - metabolism Serine Proteinase Inhibitors - biosynthesis Serine Proteinase Inhibitors - chemistry Serine Proteinase Inhibitors - pharmacology
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The structure of propeptin, a new inhibitor of prolyl endopeptidase isolated from Microbispora sp. SNA-115, was determined. FAB/MS, Edman degradation and amino acid analysis revealed propeptin to be a cyclic polypeptide consisting of 19 common L-amino acids. By FAB/MS and protein chemical methods, the primary sequence of propeptin was determined to be Gly1-Tyr-Pro-Trp-Trp-Asp-Tyr-Arg-Asp9-Leu-Phe-Gly-Gly-His-Thr-Phe-Ile-Ser-Pro19, which cyclizes between the β-carboxyl group of Asp9 and the α-amino group of Gly1.
ISSN:	0021-8820 1881-1469
DOI:	10.7164/antibiotics.55.296