A simple method for focally delivering multiple drugs or ligands to cells

A simple method for focally delivering multiple drugs or ligands to cells

A simple venturi-based ‘spritzer’ device is described that is capable of repeatedly and rapidly delivering small volumes of different solutions to cells or tissues. This is particularly advantageous in situations when the appropriate ligand or drug is not known a priori and has to be determined ‘on...

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Veröffentlicht in:Journal of neuroscience methods 2002-04, Vol.116 (1), p.9-14
Hauptverfasser: Doolin, Richard E, Ache, Barry W
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Biochemistry. Physiology. Immunology
Biological and medical sciences
Crustacea
Fundamental and applied biological sciences. Psychology
Invertebrates
Ligands
Membrane Potentials - drug effects
Membrane Potentials - physiology
Microinjections - instrumentation
Microinjections - methods
Nephropidae
Neurons
Odorants
Olfactory
Olfactory Receptor Neurons - physiology
Patch-Clamp Techniques
Physiology. Development
Proline - pharmacology
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Beschreibung
Zusammenfassung:A simple venturi-based ‘spritzer’ device is described that is capable of repeatedly and rapidly delivering small volumes of different solutions to cells or tissues. This is particularly advantageous in situations when the appropriate ligand or drug is not known a priori and has to be determined ‘on the fly’ during the limited lifetime of a preparation. The utility of the device is demonstrated for applying odorants to olfactory receptor neurons (ORNs).
ISSN:0165-0270
1872-678X
DOI:10.1016/S0165-0270(02)00024-9