Localized interleukin-10 gene transfer induces apoptosis of alloreactive T cells via Fas/FasL pathway, improves function, and prolongs survival of cardiac allograft

We hypothesized that localized IL-10 gene transfer can induce alloreactive T cell apoptosis and tested this hypothesis with liposome-mediated ex vivo intracoronary IL-10 gene transfer using a functional heterotopic allograft heart transplant model in rabbits. Localized IL-10 overexpression prolonged...

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Veröffentlicht in:	Transplantation 2002-04, Vol.73 (7), p.1019-1026
Hauptverfasser:	OSHIMA, Kiyohiro, LUYI SEN, GUANGGEN CUI, TUNG, Thomas, SACKS, Benjamin M, ARELLANO-KRUSE, Anthony, LAKS, Hillel
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Apoptosis Biological and medical sciences Caspase 3 Caspases - physiology Fas Ligand Protein fas Receptor - physiology Genetic Therapy Graft Rejection Graft Survival Heart Transplantation In Situ Nick-End Labeling Interleukin-10 - genetics Medical sciences Membrane Glycoproteins - physiology Rabbits Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Surgery of the heart T-Lymphocytes - physiology Transfection Transplantation, Homologous
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Zusammenfassung:	We hypothesized that localized IL-10 gene transfer can induce alloreactive T cell apoptosis and tested this hypothesis with liposome-mediated ex vivo intracoronary IL-10 gene transfer using a functional heterotopic allograft heart transplant model in rabbits. Localized IL-10 overexpression prolonged cardiac allograft survival over three folds. In parallel with the time-course of IL-10 overexpression, the percentage of apoptotic CD3+ cells among total CD3+ cells was significantly increased in the gene therapy group (36.5+/-3.9%) compared with that in the control group (6.2+/-2.6%, P
ISSN:	0041-1337 1534-6080
DOI:	10.1097/00007890-200204150-00002