In vitro protein refolding by chromatographic procedures

In vitro protein refolding by chromatographic procedures

In vitro protein refolding is still a bottleneck in both structural biology and in the development of new biopharmaceuticals, especially for commercially important polypeptides that are overexpressed in Escherichia coli. This review focuses on protein refolding methods based on column procedures bec...

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Veröffentlicht in:Protein Expression and Purification 2004, Vol.33 (1), p.1-10
Hauptverfasser: Li, Ming, Su, Zhi-Guo, Janson, Jan-Christer
Format: Artikel
Sprache:eng
Schlagworte:
Chaperonins - chemistry
Chromatographic techniques
Chromatography, Gel - methods
Escherichia coli - metabolism
Gradient techniques
Humans
In vitro protein refolding
Inclusion bodies
Inclusion Bodies - metabolism
Liposomes - chemistry
Polyethylene Glycols - chemistry
Protein Folding
Protein Renaturation
Urea - chemistry
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Beschreibung
Zusammenfassung:In vitro protein refolding is still a bottleneck in both structural biology and in the development of new biopharmaceuticals, especially for commercially important polypeptides that are overexpressed in Escherichia coli. This review focuses on protein refolding methods based on column procedures because recent advances in chromatographic refolding have shown promising results.
ISSN:1046-5928
1096-0279
DOI:10.1016/j.pep.2003.08.023