Detection of Encephalitis Viruses in Mosquitoes (Diptera: Culicidae) and Avian Tissues

Diagnostic assays for the detection of St. Louis encephalitis (SLE) and western equine encephalomyelitis (WEE) viruses in mosquito pools and avian tissues were compared for sensitivity, accuracy and specificity. The in situ enzyme immunoassay (EIA), plaque assay on Vero cells, passage in Aedes albop...

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Veröffentlicht in:	Journal of medical entomology 2002-03, Vol.39 (2), p.312-323
Hauptverfasser:	Kramer, L. D., Wolfe, T. M., Green, E. N., Chiles, R. E., Fallah, H., Fang, Y., Reisen, W. K.
Format:	Artikel
Sprache:	eng
Schlagworte:	Aedes - virology Animals arboviruses Asian tiger mosquito Biological and medical sciences Bird Diseases - pathology Bird Diseases - virology Birds Cercopithecus aethiops Culex - virology DNA, Viral - analysis Encephalitis Encephalitis Virus, St. Louis - genetics Encephalitis Virus, St. Louis - immunology Encephalitis Virus, St. Louis - isolation & purification Encephalitis Virus, Western Equine - genetics Encephalitis Virus, Western Equine - immunology Encephalitis Virus, Western Equine - isolation & purification Encephalitis, St. Louis - pathology Encephalitis, St. Louis - veterinary Encephalitis, St. Louis - virology Encephalomyelitis, Western Equine - pathology Encephalomyelitis, Western Equine - veterinary Encephalomyelitis, Western Equine - virology enzyme immunoassay Enzymes Female Fundamental and applied biological sciences. Psychology Health aspects Medically important nuisances and vectors, pests of stored products and materials: population survey and control Mosquitoes reverse transcription-polymerase chain reaction RNA Sensitivity and Specificity Songbirds - virology surveillance Vectors. Intermediate hosts Vero Cells virus isolation
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Beschreibung
Zusammenfassung:	Diagnostic assays for the detection of St. Louis encephalitis (SLE) and western equine encephalomyelitis (WEE) viruses in mosquito pools and avian tissues were compared for sensitivity, accuracy and specificity. The in situ enzyme immunoassay (EIA), plaque assay on Vero cells, passage in Aedes albopictus Skuse C6/36 and C7/10 cells, antigen capture enzyme immunoassay (AC-EIA), and single and multiplex reverse transcription-polymerase chain reactions (RT-PCR) were evaluated using pools of 50 mosquitoes containing 1–2 experimentally infected individuals. RT-PCR was the most sensitive assay, with a detection limit of
ISSN:	0022-2585 1938-2928
DOI:	10.1603/0022-2585-39.2.312