Transverse fluctuation analysis of single extended DNA molecules

We observed fluctuations of elongated DNA molecules by fluorescence microscopy. The molecules are fixed at both ends and undulate. Mode analysis of the thermally excited undulations of the labeled DNA molecules gives access to the spectral density of the amplitude fluctuations. From these measuremen...

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Veröffentlicht in:	The European physical journal. E, Soft matter and biological physics Soft matter and biological physics, 2003-05, Vol.11 (1), p.105-108
Hauptverfasser:	GUEROUI, Z, FREYSSINGEAS, E, PLACE, C, BERGE, B
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Computer Simulation DNA, Viral - chemistry DNA, Viral - ultrastructure Elasticity Fundamental and applied biological sciences. Psychology Image Interpretation, Computer-Assisted - methods Micromanipulation - methods Microscopy, Fluorescence - methods Miscellaneous Models, Molecular Molecular biophysics Nucleic Acid Conformation Physical Stimulation - methods Physico-chemical properties of biomolecules Stress, Mechanical
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Beschreibung
Zusammenfassung:	We observed fluctuations of elongated DNA molecules by fluorescence microscopy. The molecules are fixed at both ends and undulate. Mode analysis of the thermally excited undulations of the labeled DNA molecules gives access to the spectral density of the amplitude fluctuations. From these measurements we estimate the tension acting on the DNA molecules. We found the forces to be within the entropic elasticity range of a typical DNA molecule (measured on the single-molecule level).
ISSN:	1292-8941 1292-895X
DOI:	10.1140/epje/i2002-10150-9