Cryopreservation of cultivated and wild arachis species embryonic axes using desiccation and vitrification methods

The effects of two methods of cryopreservation involving chemical vitrification and air desiccation) were studied on isolated embryonic axes of A. hypogaea. Vitrification with PVS2 and desiccation in a laminar flow cabinet resulted in high levels (70-90%) of whole plant recovery after cryopreservati...

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Veröffentlicht in:	Cryo-Letters 2002-01, Vol.23 (1), p.61-68
Hauptverfasser:	Gagliardi, R F, Pacheco, G P, Valls, J F M, Mansur, E
Format:	Artikel
Sprache:	eng
Schlagworte:	Arachis - embryology Cryopreservation Cryopreservation - methods Cryoprotective Agents - pharmacology Desiccation Desiccation - methods Dimethyl Sulfoxide - pharmacology Extranervosae Germplasm Preservation Glycerol - pharmacology Groundnut Nitrogen Peanut Plant Shoots - growth & development Recovery of Function Seeds - drug effects Seeds - growth & development Sucrose - pharmacology
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Beschreibung
Zusammenfassung:	The effects of two methods of cryopreservation involving chemical vitrification and air desiccation) were studied on isolated embryonic axes of A. hypogaea. Vitrification with PVS2 and desiccation in a laminar flow cabinet resulted in high levels (70-90%) of whole plant recovery after cryopreservation. A desiccation protocol based on 1h exposure of explants to the air flow was successfully applied to six wild species of section Extranervosae, resulting in recovery levels of 70-90% after liquid nitrogen treatment.
ISSN:	0143-2044