Effect of moxifloxacin on secretion of cytokines by human monocytes stimulated with lipopolysaccharide
Objective To determine the effect of moxifloxacin on secretion of cytokines by human monocytes stimulated with lipopolysaccharide (LPS) or Pansorbin. Methods Monocytes obtained from 10 healthy volunteer donors were stimulated with LPS or Pansorbin and exposed or not to different concentrations of th...
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| Veröffentlicht in: | Clinical microbiology and infection 2002-01, Vol.8 (1), p.26-30 |
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| creator | Araujo, F.G. Slifer, T.L. Remington, J.S. |
| description | Objective To determine the effect of moxifloxacin on secretion of cytokines by human monocytes stimulated with lipopolysaccharide (LPS) or Pansorbin.
Methods Monocytes obtained from 10 healthy volunteer donors were stimulated with LPS or Pansorbin and exposed or not to different concentrations of the fluoroquinolone antibiotic moxifloxacin. At 3, 6 and 24 h, the amounts of interleukin-1α (IL-1α), IL-1β, IL-6, IL-10, IL-12 (p70) and tumour necrosis factor-α (TNF-α) were measured in the supernatants of the monocyte cultures using enzyme-linked immunosorbent assay.
Results Stimulation of human monocytes with either LPS or Pansorbin resulted in a significant increase in secretion of each of the cytokines examined. Treatment of LPS-stimulated monocytes with moxifloxacin significantly inhibited (P |
| doi_str_mv | 10.1046/j.1469-0691.2002.00374.x |
| format | Article |
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Methods Monocytes obtained from 10 healthy volunteer donors were stimulated with LPS or Pansorbin and exposed or not to different concentrations of the fluoroquinolone antibiotic moxifloxacin. At 3, 6 and 24 h, the amounts of interleukin-1α (IL-1α), IL-1β, IL-6, IL-10, IL-12 (p70) and tumour necrosis factor-α (TNF-α) were measured in the supernatants of the monocyte cultures using enzyme-linked immunosorbent assay.
Results Stimulation of human monocytes with either LPS or Pansorbin resulted in a significant increase in secretion of each of the cytokines examined. Treatment of LPS-stimulated monocytes with moxifloxacin significantly inhibited (P<0.01) secretion of IL-1α by monocytes of each of 10 human donors; the secretion of TNF-α was significantly inhibited (P<0.01) in monocytes from six of 10 donors. In general there was a trend towards inhibition of secretion of IL-6, IL-10 and IL-12 (p70), but the inhibitory effect was not statistically significant. Secretion of cytokines by Pansorbin-stimulated monocytes was not significantly inhibited by moxifloxacin.
Conclusions Moxifloxacin has immunomodulatory activity through its capacity to alter the secretion of IL-1α and TNF-α by human monocytes.</description><identifier>ISSN: 1198-743X</identifier><identifier>EISSN: 1469-0691</identifier><identifier>DOI: 10.1046/j.1469-0691.2002.00374.x</identifier><identifier>PMID: 11906497</identifier><language>eng</language><publisher>Oxford, UK: Elsevier Ltd</publisher><subject>Adjuvants, Immunologic - pharmacology ; Adult ; Anti-Infective Agents - pharmacology ; Antibacterial agents ; Antibiotics. Antiinfectious agents. Antiparasitic agents ; Aza Compounds ; Biological and medical sciences ; cytokines ; Escherichia coli ; Female ; Fluoroquinolones ; Humans ; Interleukin-1 - secretion ; Lipopolysaccharides - immunology ; Male ; Medical sciences ; Middle Aged ; monocytes ; Monocytes - drug effects ; Monocytes - immunology ; Monocytes - secretion ; moxifloxacin ; Pharmacology. Drug treatments ; Quinolines ; Staphylococcus aureus ; Tumor Necrosis Factor-alpha - secretion</subject><ispartof>Clinical microbiology and infection, 2002-01, Vol.8 (1), p.26-30</ispartof><rights>2002 European Society of Clinical Infectious Diseases</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5364-40f2a907cb5dac052d35e768af2606b9725bbb863a4b24bc42202931087f4eb03</citedby><cites>FETCH-LOGICAL-c5364-40f2a907cb5dac052d35e768af2606b9725bbb863a4b24bc42202931087f4eb03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1469-0691.2002.00374.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1469-0691.2002.00374.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,4024,27923,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13606359$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11906497$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Araujo, F.G.</creatorcontrib><creatorcontrib>Slifer, T.L.</creatorcontrib><creatorcontrib>Remington, J.S.</creatorcontrib><title>Effect of moxifloxacin on secretion of cytokines by human monocytes stimulated with lipopolysaccharide</title><title>Clinical microbiology and infection</title><addtitle>Clin Microbiol Infect</addtitle><description>Objective To determine the effect of moxifloxacin on secretion of cytokines by human monocytes stimulated with lipopolysaccharide (LPS) or Pansorbin.
Methods Monocytes obtained from 10 healthy volunteer donors were stimulated with LPS or Pansorbin and exposed or not to different concentrations of the fluoroquinolone antibiotic moxifloxacin. At 3, 6 and 24 h, the amounts of interleukin-1α (IL-1α), IL-1β, IL-6, IL-10, IL-12 (p70) and tumour necrosis factor-α (TNF-α) were measured in the supernatants of the monocyte cultures using enzyme-linked immunosorbent assay.
Results Stimulation of human monocytes with either LPS or Pansorbin resulted in a significant increase in secretion of each of the cytokines examined. Treatment of LPS-stimulated monocytes with moxifloxacin significantly inhibited (P<0.01) secretion of IL-1α by monocytes of each of 10 human donors; the secretion of TNF-α was significantly inhibited (P<0.01) in monocytes from six of 10 donors. In general there was a trend towards inhibition of secretion of IL-6, IL-10 and IL-12 (p70), but the inhibitory effect was not statistically significant. Secretion of cytokines by Pansorbin-stimulated monocytes was not significantly inhibited by moxifloxacin.
Conclusions Moxifloxacin has immunomodulatory activity through its capacity to alter the secretion of IL-1α and TNF-α by human monocytes.</description><subject>Adjuvants, Immunologic - pharmacology</subject><subject>Adult</subject><subject>Anti-Infective Agents - pharmacology</subject><subject>Antibacterial agents</subject><subject>Antibiotics. Antiinfectious agents. Antiparasitic agents</subject><subject>Aza Compounds</subject><subject>Biological and medical sciences</subject><subject>cytokines</subject><subject>Escherichia coli</subject><subject>Female</subject><subject>Fluoroquinolones</subject><subject>Humans</subject><subject>Interleukin-1 - secretion</subject><subject>Lipopolysaccharides - immunology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>monocytes</subject><subject>Monocytes - drug effects</subject><subject>Monocytes - immunology</subject><subject>Monocytes - secretion</subject><subject>moxifloxacin</subject><subject>Pharmacology. Drug treatments</subject><subject>Quinolines</subject><subject>Staphylococcus aureus</subject><subject>Tumor Necrosis Factor-alpha - secretion</subject><issn>1198-743X</issn><issn>1469-0691</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkcuO1DAQRSMEYh7wC8gbEJsEv-IkEhumNQxIjdiAxM6ynbLajRM3dsJ0_h6HbjE7YOWS69yy696iQARXBHPxZl8RLroSi45UFGNaYcwaXh0fFZd_Go9zTbq2bDj7dlFcpbTHmWSMPy0ucgML3jWXhb21FsyEgkVDODrrw1EZN6IwogQmwuRylZtmmcJ3N0JCekG7eVBj5seQr_NVmtwwezVBj-7dtEPeHcIh-CUpY3Yquh6eFU-s8gmen8_r4uv72y-bD-X2893HzbttaWomeMmxparDjdF1rwyuac9qaESrLBVY6K6htda6FUxxTbk2nFJMO0Zw21gOGrPr4tVp7iGGHzOkSQ4uGfBejRDmJBtSM9oSksHXfwVJLUTLKaHrzPaEmhhSimDlIbpBxUUSLNc45F6ursvVdbnGIX_HIY9Z-uL8yqwH6B-EZ_8z8PIMqGSUt1GNxqUHjuW1Wd1l7u2Ju3celv_-gNxsP-Uiy29Ocsje_3QQZTIORgO9izl92Qf372V-AR90u8o</recordid><startdate>200201</startdate><enddate>200201</enddate><creator>Araujo, F.G.</creator><creator>Slifer, T.L.</creator><creator>Remington, J.S.</creator><general>Elsevier Ltd</general><general>Blackwell Science, Ltd</general><general>Blackwell</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>200201</creationdate><title>Effect of moxifloxacin on secretion of cytokines by human monocytes stimulated with lipopolysaccharide</title><author>Araujo, F.G. ; Slifer, T.L. ; Remington, J.S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5364-40f2a907cb5dac052d35e768af2606b9725bbb863a4b24bc42202931087f4eb03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Adjuvants, Immunologic - pharmacology</topic><topic>Adult</topic><topic>Anti-Infective Agents - pharmacology</topic><topic>Antibacterial agents</topic><topic>Antibiotics. Antiinfectious agents. Antiparasitic agents</topic><topic>Aza Compounds</topic><topic>Biological and medical sciences</topic><topic>cytokines</topic><topic>Escherichia coli</topic><topic>Female</topic><topic>Fluoroquinolones</topic><topic>Humans</topic><topic>Interleukin-1 - secretion</topic><topic>Lipopolysaccharides - immunology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>monocytes</topic><topic>Monocytes - drug effects</topic><topic>Monocytes - immunology</topic><topic>Monocytes - secretion</topic><topic>moxifloxacin</topic><topic>Pharmacology. Drug treatments</topic><topic>Quinolines</topic><topic>Staphylococcus aureus</topic><topic>Tumor Necrosis Factor-alpha - secretion</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Araujo, F.G.</creatorcontrib><creatorcontrib>Slifer, T.L.</creatorcontrib><creatorcontrib>Remington, J.S.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical microbiology and infection</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Araujo, F.G.</au><au>Slifer, T.L.</au><au>Remington, J.S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of moxifloxacin on secretion of cytokines by human monocytes stimulated with lipopolysaccharide</atitle><jtitle>Clinical microbiology and infection</jtitle><addtitle>Clin Microbiol Infect</addtitle><date>2002-01</date><risdate>2002</risdate><volume>8</volume><issue>1</issue><spage>26</spage><epage>30</epage><pages>26-30</pages><issn>1198-743X</issn><eissn>1469-0691</eissn><abstract>Objective To determine the effect of moxifloxacin on secretion of cytokines by human monocytes stimulated with lipopolysaccharide (LPS) or Pansorbin.
Methods Monocytes obtained from 10 healthy volunteer donors were stimulated with LPS or Pansorbin and exposed or not to different concentrations of the fluoroquinolone antibiotic moxifloxacin. At 3, 6 and 24 h, the amounts of interleukin-1α (IL-1α), IL-1β, IL-6, IL-10, IL-12 (p70) and tumour necrosis factor-α (TNF-α) were measured in the supernatants of the monocyte cultures using enzyme-linked immunosorbent assay.
Results Stimulation of human monocytes with either LPS or Pansorbin resulted in a significant increase in secretion of each of the cytokines examined. Treatment of LPS-stimulated monocytes with moxifloxacin significantly inhibited (P<0.01) secretion of IL-1α by monocytes of each of 10 human donors; the secretion of TNF-α was significantly inhibited (P<0.01) in monocytes from six of 10 donors. In general there was a trend towards inhibition of secretion of IL-6, IL-10 and IL-12 (p70), but the inhibitory effect was not statistically significant. Secretion of cytokines by Pansorbin-stimulated monocytes was not significantly inhibited by moxifloxacin.
Conclusions Moxifloxacin has immunomodulatory activity through its capacity to alter the secretion of IL-1α and TNF-α by human monocytes.</abstract><cop>Oxford, UK</cop><pub>Elsevier Ltd</pub><pmid>11906497</pmid><doi>10.1046/j.1469-0691.2002.00374.x</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Access via Wiley Online Library; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Adjuvants, Immunologic - pharmacology Adult Anti-Infective Agents - pharmacology Antibacterial agents Antibiotics. Antiinfectious agents. Antiparasitic agents Aza Compounds Biological and medical sciences cytokines Escherichia coli Female Fluoroquinolones Humans Interleukin-1 - secretion Lipopolysaccharides - immunology Male Medical sciences Middle Aged monocytes Monocytes - drug effects Monocytes - immunology Monocytes - secretion moxifloxacin Pharmacology. Drug treatments Quinolines Staphylococcus aureus Tumor Necrosis Factor-alpha - secretion |
| title | Effect of moxifloxacin on secretion of cytokines by human monocytes stimulated with lipopolysaccharide |
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