Effect of moxifloxacin on secretion of cytokines by human monocytes stimulated with lipopolysaccharide

Objective To determine the effect of moxifloxacin on secretion of cytokines by human monocytes stimulated with lipopolysaccharide (LPS) or Pansorbin. Methods Monocytes obtained from 10 healthy volunteer donors were stimulated with LPS or Pansorbin and exposed or not to different concentrations of the fluoroquinolone antibiotic moxifloxacin. At 3, 6 and 24 h, the amounts of interleukin-1α (IL-1α), IL-1β, IL-6, IL-10, IL-12 (p70) and tumour necrosis factor-α (TNF-α) were measured in the supernatants of the monocyte cultures using enzyme-linked immunosorbent assay. Results Stimulation of human monocytes with either LPS or Pansorbin resulted in a significant increase in secretion of each of the cytokines examined. Treatment of LPS-stimulated monocytes with moxifloxacin significantly inhibited (P