Neural cell fate analysis in zebrafish using olig2 BAC transgenics

We describe here simple methods for producing transgenic zebrafish reporter lines using BAC clones. The use of BAC clones facilitates creation of useful transgenics as the large amounts of genomic DNA they contain increase the likelihood that reporter gene expression will be properly regulated. Comb...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Methods in cell science 2003, Vol.25 (1-2), p.7-14
Hauptverfasser:	Shin, Jimann, Park, Hae-Chul, Topczewska, Jolanta M, Mawdsley, David J, Appel, Bruce
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cloning, Molecular Danio rerio Embryo, Nonmammalian - cytology Embryo, Nonmammalian - metabolism Freshwater Gene Expression Regulation, Developmental - physiology Genes, Reporter - genetics Genetic Vectors Green Fluorescent Proteins Luminescent Proteins - metabolism Microinjections Motor Neurons - cytology Motor Neurons - metabolism Peripheral Nervous System - cytology Peripheral Nervous System - metabolism Purkinje Cells - cytology Purkinje Cells - metabolism Zebrafish - anatomy & histology Zebrafish - genetics Zebrafish - metabolism
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	We describe here simple methods for producing transgenic zebrafish reporter lines using BAC clones. The use of BAC clones facilitates creation of useful transgenics as the large amounts of genomic DNA they contain increase the likelihood that reporter gene expression will be properly regulated. Combined with recent advances in live embryo image analysis, this strategy has the potential to greatly advance the investigation of neural cell behavior during development.
ISSN:	1381-5741
DOI:	10.1023/b:mics.0000006847.09037.3a