Cellular cardiomyoplasty in a transgenic mouse model

Recent progress in the cardiotypic differentiation of embryonic and somatic stem cells opens novel prospects for the treatment of cardiovascular disorders. The aim of the present study was to develop a novel surgical approach that allows standardized cellular cardiomyoplasty in mouse with low-periop...

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Veröffentlicht in:Transplantation 2002-02, Vol.73 (3), p.462-465
Hauptverfasser: ROELL, Wilhelm, YUN FAN, HESCHELER, Juergen, WELZ, Armin, FLEISCHMANN, Bernd K, YING XIA, STOECKER, Eva, SASSE, Philipp, KOLOSSOV, Eugen, BLOCH, Wilhelm, METZNER, Harald, SCHMITZ, Christoph, ADDICKS, Klaus
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Sprache:eng
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Zusammenfassung:Recent progress in the cardiotypic differentiation of embryonic and somatic stem cells opens novel prospects for the treatment of cardiovascular disorders. The aim of the present study was to develop a novel surgical approach that allows standardized cellular cardiomyoplasty in mouse with low-perioperative mortality. Reproducible transmural lesions were generated by cryoinjury followed by intramural injection of embryonic cardiomyocytes using a newly designed holding device and vital dye staining. This approach was validated with a transgenic mouse model, in which the live reporter gene-enhanced green fluorescent protein (EGFP) is under control of a cardiac-specific promoter. The perioperative mortality was 10%. The engrafted EGFP-positive cardiomyocytes could be identified in a high percentage (72.2%, n=36) of operated animals. This novel approach enables reliable cellular replacement therapy in mouse and greatly facilitates the analysis of its molecular, cellular, and functional efficacy.
ISSN:0041-1337
1534-6080
DOI:10.1097/00007890-200202150-00022