Targeted Proteomic Profiling of Renal Na+ Transporter and Channel Abundances in Angiotensin II Type 1a Receptor Knockout Mice

The renal tubule transporters responsible for Na and water transport along the nephron have been identified and cloned, permitting comprehensive analysis of transporter protein abundance changes in complex physiological models by using a “targeted proteomics” approach. Here, we apply this approach to screen renal homogenates from mice in which the gene for the angiotensin II type 1a (AT1a) receptor has been deleted (versus wild-type mice) to determine which sodium transporters and channels are regulated by the AT1a receptor at the protein abundance level. In mice maintained on a low NaCl diet (