Luminous plant and animals or the expression of aequorin and "chameleon" probes: a new light in calcium signaling

Calcium ion is a universal second messenger in numerous cell physiological processes. The paper describes the structure and the activation mechanisms of the bioluminescent (aequorin) and fluorescent based GFP calcium sensitive probes (Cameleon) and the data obtained with such probes in genetically t...

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Veröffentlicht in:	Journal de la Société de biologie 2003, Vol.197 (3), p.291-300
Hauptverfasser:	Thuleau, Patrice, Leclerc, Catherine, Xiong, Tou-Cheu, Mazars, Christian, Moreau, Marc
Format:	Artikel
Sprache:	fre
Schlagworte:	Aequorin - metabolism Animals Calcium Signaling - physiology Green Fluorescent Proteins Luminescent Proteins - metabolism Plant Physiological Phenomena Plant Proteins - metabolism
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	Calcium ion is a universal second messenger in numerous cell physiological processes. The paper describes the structure and the activation mechanisms of the bioluminescent (aequorin) and fluorescent based GFP calcium sensitive probes (Cameleon) and the data obtained with such probes in genetically transformed animal and vegetal organisms. The importance of these in vivo Ca2+ imaging molecules in the understanding of calcium signalling is discussed.
ISSN:	1295-0661