Thermostable Pyrococcus furiosus DNA ligase catalyzes the synthesis of (di)nucleoside polyphosphates

DNA ligase from the hyperthermophilic marine archaeon Pyrococcus furiosus (Pfu DNA ligase) synthesizes adenosine 5'-tetraphosphate (p4A) and dinucleoside polyphosphates by displacement of the adenosine 5'-monophosphate (AMP) from the Pfu DNA ligase-AMP (E-AMP) complex with tripolyphosphate...

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Veröffentlicht in:	Extremophiles : life under extreme conditions 2002-02, Vol.6 (1), p.45-50
Hauptverfasser:	Günther, SilleroMariaA, Montes, María, de, Diego Anabel, del, Valle Mercedes, Atencia, Eva Ana, Sillero, Antonio
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Monophosphate - metabolism Catalysis Deoxyribonucleotides - metabolism Dinucleoside Phosphates - biosynthesis DNA Ligases - metabolism Kinetics Pyrococcus furiosus - enzymology Ribonucleotides - metabolism Space life sciences Substrate Specificity Thermodynamics
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Beschreibung
Zusammenfassung:	DNA ligase from the hyperthermophilic marine archaeon Pyrococcus furiosus (Pfu DNA ligase) synthesizes adenosine 5'-tetraphosphate (p4A) and dinucleoside polyphosphates by displacement of the adenosine 5'-monophosphate (AMP) from the Pfu DNA ligase-AMP (E-AMP) complex with tripolyphosphate (P3), nucleoside triphosphates (NTP), or nucleoside diphosphates (NDP). The experiments were performed in the presence of 1-2 microM [alpha-32P]ATP and millimolar concentrations of NTP or NDP. Relative rates of synthesis (%) of the following adenosine(5')tetraphospho(5')nucleosides (Ap4N) were observed: Ap4guanosine (Ap4G) (from GTP, 100); Ap4deoxythymidine (Ap4dT) (from dTTP, 95); Ap4xanthosine (Ap4X) (from XTP, 94); Ap4deoxycytidine (Ap4dC) (from dCTP, 64); Ap4cytidine (Ap4C) (from CTP, 60); Ap4deoxyguanosine (Ap4dG) (from dGTP, 58); Ap4uridine (Ap4U) (from UTP,
ISSN:	1431-0651 1433-4909
DOI:	10.1007/s007920100227