Long‐term survival of Escherichia coli O157 on pasture following an outbreak associated with sheep at a scout camp

Aims: To monitor the decay of E. coli O157 in soil (loamy sand) on a scout campsite following an outbreak in humans. Methods and Results: Samples of soil and sheep faeces were collected from the campsite and tested for the presence of E. coli O157 by immunomagnetic separation (IMS) after enrichment...

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Veröffentlicht in:	Letters in applied microbiology 2002-01, Vol.34 (2), p.100-104
Hauptverfasser:	Ogden, I.D., Hepburn, N.F., MacRae, M., Strachan, N.J.C., Fenlon, D.R., Rusbridge, S.M., Pennington, T.H.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Disease Outbreaks Escherichia coli Escherichia coli Infections - epidemiology Escherichia coli Infections - microbiology Escherichia coli Infections - veterinary Escherichia coli O157 - growth & development Escherichia coli O157 - isolation & purification Feces - microbiology Humans Immunomagnetic Separation Sheep Sheep Diseases - microbiology Soil Microbiology Time Factors
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Zusammenfassung:	Aims: To monitor the decay of E. coli O157 in soil (loamy sand) on a scout campsite following an outbreak in humans. Methods and Results: Samples of soil and sheep faeces were collected from the campsite and tested for the presence of E. coli O157 by immunomagnetic separation (IMS) after enrichment in buffered peptone water + vancomycin at 42°C for 6 h. Enumeration of target was carried out by direct plating onto sorbitol MacConkey agar plates supplemented with cefixime and tellurite (CTSMAC) incubated at 37°C for 24 h. Low numbers (
ISSN:	0266-8254 1472-765X 1365-2673
DOI:	10.1046/j.1472-765x.2002.01052.x