Expression and Cellular Localization of Classic NADPH Oxidase Subunits in the Spontaneously Hypertensive Rat Kidney

Phagocytes generate superoxide anion (O2) by a classic, 5-component NADPH oxidase. O2 contributes to hypertension in spontaneously hypertensive rats (SHR). Therefore, we tested the hypothesis that NADPH oxidase expression is enhanced in the SHR kidney. We also analyzed the localization of NADPH oxid...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Hypertension (Dallas, Tex. 1979) Tex. 1979), 2002-02, Vol.39 (2), p.269-274
Hauptverfasser:	Chabrashvili, Tinatin, Tojo, Akahiro, Onozato, Maristela Lika, Kitiyakara, Chagriya, Quinn, Mark T, Fujita, Toshiro, Welch, William J, Wilcox, Christopher S
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Arterial hypertension. Arterial hypotension Biological and medical sciences Blood and lymphatic vessels Blotting, Western Cardiology. Vascular system Experimental diseases Gene Expression Regulation, Enzymologic Hypertension - enzymology Hypertension - genetics Immunohistochemistry Kidney - enzymology Male Medical sciences Membrane Transport Proteins NADPH Dehydrogenase - genetics NADPH Dehydrogenase - metabolism NADPH Oxidases - genetics NADPH Oxidases - metabolism Phosphoproteins - genetics Phosphoproteins - metabolism Protein Subunits Rats Rats, Inbred SHR Rats, Inbred WKY Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - genetics RNA, Messenger - metabolism
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Phagocytes generate superoxide anion (O2) by a classic, 5-component NADPH oxidase. O2 contributes to hypertension in spontaneously hypertensive rats (SHR). Therefore, we tested the hypothesis that NADPH oxidase expression is enhanced in the SHR kidney. We also analyzed the localization of NADPH oxidase components in SHR kidney. Renal NADPH oxidase was quantified by reverse transcription-polymerase chain reaction and Western blotting and was localized in SHR and Wistar Kyoto rat (WKY) kidney by immunohistochemistry. The mRNA for 5 subunits of phagocyte NADPH oxidase, and also for MOX1 and RENOX (NOX4), was detected in adult rat kidney. Kidneys of adult (10 weeks old) SHR had a significantly (P
ISSN:	0194-911X 1524-4563
DOI:	10.1161/hy0202.103264