An additional 2′-ribofuranose residue at a specific position of the DNA primer prevents Its elongation by HIV-1 reverse transcriptase

Oligodeoxynucleotides containing 2′- O-β- d-ribofuranosyladenosine were prepared and used as modified primers in RNA-templated DNA synthesis catalyzed by HIV reverse transcriptase. It was shown that the additional 2′-ribofuranose residue in specific position of primer prevents its elongation. 5′-dGXC-GTT-GTX-XXX-CG-3′ (X=dAdo or 2′- O-β- d-ribofuranosy1adenosine residues) were prepared and used as modified primers in DNA synthesis reaction catalyzing by HIV RT on RNA template.