Acetylsalicylic acid reduces heat responses in rat nociceptive primary sensory neurons – evidence for a new mechanism of action

Acetylsalicylic acid (ASA) is thought to exert its peripheral analgesic effects via inhibition of cyclooxygenase. We now studied the effects of ASA on heat responses in primary nociceptive neurons by whole-cell patch-clamp and calcium microfluorimetry experiments. Heat-evoked inward currents in acut...

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Veröffentlicht in:	Neuroscience letters 2002-03, Vol.320 (1), p.61-64
Hauptverfasser:	Greffrath, Wolfgang, Kirschstein, Timo, Nawrath, Hermann, Treede, Rolf-Detlef
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Aspirin Aspirin - pharmacology Biological and medical sciences Bones, joints and connective tissue. Antiinflammatory agents Calcium Calcium - metabolism Calcium Signaling - drug effects Calcium Signaling - physiology Capsaicin Capsaicin - pharmacology Cells, Cultured Cyclooxygenase Cyclooxygenase Inhibitors - pharmacology Dose-Response Relationship, Drug Electric Stimulation Ganglia, Spinal - cytology Ganglia, Spinal - drug effects Ganglia, Spinal - metabolism Heat transduction Hot Temperature Ion Channels - drug effects Ion Channels - metabolism Male Medical sciences Membrane Potentials - drug effects Membrane Potentials - physiology Neurons, Afferent - cytology Neurons, Afferent - drug effects Neurons, Afferent - metabolism Nociceptors Nociceptors - cytology Nociceptors - drug effects Nociceptors - metabolism Pain - drug therapy Pain - metabolism Pain - physiopathology Patch-clamp Patch-Clamp Techniques Pharmacology. Drug treatments Rats Rats, Sprague-Dawley Signal Transduction - drug effects Signal Transduction - physiology Thermosensing - drug effects Thermosensing - physiology Vanilloid receptors
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Zusammenfassung:	Acetylsalicylic acid (ASA) is thought to exert its peripheral analgesic effects via inhibition of cyclooxygenase. We now studied the effects of ASA on heat responses in primary nociceptive neurons by whole-cell patch-clamp and calcium microfluorimetry experiments. Heat-evoked inward currents in acutely dissociated rat dorsal root ganglion neurons were significantly reduced by ASA in a dose-dependent and reversible manner (IC 50 375 nM, Hill slope −2.2, maximum effect 55%). Heat-evoked calcium transients (measured with FURA-2) were reversibly reduced by 53±14% ( P
ISSN:	0304-3940 1872-7972
DOI:	10.1016/S0304-3940(02)00033-2