Gradual evolution in bacteria: evidence from Bacillus systematics

Department of Biology, Wesleyan University, Middletown, CT 06459-0170, USA Correspondence Frederick M. Cohan fcohan{at}wesleyan.edu The bacterial genome projects have suggested a central role for horizontal transfer in bacterial adaptation, but it is difficult to rule out an adaptive role for ordinary genetic change in existing genes. The bacterial systematics literature can readily address the importance of gene acquisition in adaptive evolution, since phenotypic characterization typically assesses presence versus absence of metabolic capabilities, and metabolic gains and losses are most likely due to horizontal transfer and/or gene loss. Bacterial systematists have not geared their studies toward quantitative differences in metabolic capabilities, which are more likely to involve adjustments of existing genes. Here, quantitative variation in metabolism within and between three closely related Bacillus taxa has been assayed. While these taxa show no qualitative (i.e. presence versus absence) differences in resource utilization, they are quantitatively different in utilization of 8 % of 95 resources tested. Moreover, 93 % of the resources tested showed significant quantitative variation among strains within a single taxon. These results suggest that ordinary genetic changes in existing genes may play an important role in adaptation. If these results are typical, future genomically based assays of quantitative variation in phenotype (e.g. microarray analysis of mRNA concentrations) may identify hundreds of genes whose expression has been modified. A protocol is presented for identifying those modifications of gene expression and those gene acquisitions that are most likely to have played a role in adaptive evolution. Results of a principal component analysis for utilization of the 95 substrates examined in this study are available from Microbiology Online. Present address: Department of Ecology and Evolution, SUNY Stony Brook, Stony Brook, NY 11794-5245, USA. Present address: Department of Pediatrics and Neonatology, Box 3179, Duke University Medical Center, Durham, NC 27710, USA.