Analysis of cystine in human blood for monitoring of cases of burns

Various diseases cause abnormalities in the composition of blood and urine by increase or decrease of concentrations of its constituents or through the appearance of new compounds therein. The analysis of blood samples obtained from a healthy individual and burns patients, for its cystine contents has been done successfully using direct current poloragraphy (DCP), differential pulse polarography (DPP) and differential pulse anodic stripping voltammetry (DPASV). The observed data was analysed statistically. The standard deviation and coefficient of variance of the data proved high reliability and accuracy of the method. In Bredicka Cobaltous solution (0.001 M CoCl 2+0.1 M NH 4OH+0.1 M NH 4Cl) at pH 8.5±0.02, cystine present in blood samples produces two step catalytic hydrogen reduction wave with peak potential ( E P) values equal to −l.22 and −1.46 V vs SCE. The height of the second wave was found to be proportional to cystine concentration. Blood samples obtained from a healthy individual and those from cases of burns having different percentages (16, 13, 9 and 6%) were analysed. Monitoring of the recovery of a patient (burn case 58%) after different time intervals during the treatment period was also done, till the patient was relieved from the hospital. The method has proved to be convenient and less time consuming for the clinical purpose.