Isolation and properties of major components of Penicillium canescens extracellular enzyme complex
The composition of the enzyme complex secreted by Penicillium canescens was investigated. A scheme for purification of the main components of the complex by chromatofocusing on a Mono P column was developed. It was found that along with beta-galactosidase, the major components of the complex were en...
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Veröffentlicht in: | Biochemistry (Moscow) 2003-11, Vol.68 (11), p.1200-1209 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | The composition of the enzyme complex secreted by Penicillium canescens was investigated. A scheme for purification of the main components of the complex by chromatofocusing on a Mono P column was developed. It was found that along with beta-galactosidase, the major components of the complex were endo-beta-1,4-xylanase (31 kD, pI 8.2-9.3), alpha-L-arabinofuranosidase (60 kD, pI 7.6), arabinoxylan-arabinofuranohydrolase (70 kD, pI 3.8-4.0), and endo-beta-1,3/1,4-glucanase (40 kD, pI 4.4). The substrate specificity, pH and temperature activity optima, adsorbability, thermal stability, and ability for synergic interaction of the isolated enzymes were studied. |
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ISSN: | 0006-2979 1608-3040 |
DOI: | 10.1023/b:biry.0000009134.48246.7e |