Brain electrical activity during combined hypoxemia and hypoperfusion in anesthetized rats

In order to investigate the effects of moderate hypoxemia on brain electrical activity and the consequences of an altered cerebro-vascular response to hypoxemia, we recorded changes in electrical activity of the brain in anesthetized rats following unilateral carotid artery ligation (UCAL). In these animals, on the clamped side, cerebral blood flow, whilst normal during normoxia, shows less augmentation during hypoxemia. Six anesthetized (Halothane) Sprague–Dawley rats with UCAL were studied during 20 min periods of baseline (F i O 2 =30%), hypoxemia (F i O 2 =9.5%) and recovery (F i O 2 =30%): mean arterial pressure of oxygen (P a O 2 ) achieved was 177.0, 37.6 and 160.1 mmHg, respectively. A significant decrease in the frequencies of the ECoG was observed bilaterally during hypoxemia: centroid frequency (fc)=3.37±0.14 and 2.85±0.13 Hz on the intact and clamped hemisphere respectively during hypoxemia versus fc=4.09±0.20 Hz (mean±S.E.M.) during baseline, which was not reversed during recovery (3.27±0.11 Hz) (ANOVA, P