Cloning of Hybridoma Cell Lines by Limiting Dilution

Cloning of Hybridoma Cell Lines by Limiting Dilution

Cloning by limiting dilution is a method based on the Poisson distribution. Dilution of cells to an appropriate number per well can maximize the proportion of wells that contain one single clone. Two or more cloning procedures are carried out until >90% of the wells containing single clones are p...

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Veröffentlicht in:Current protocols in molecular biology (Print) 2001-05, Vol.1 (1), p.11.8.1-11.8.2
Hauptverfasser: Fuller, Steven A., Takahashi, Miyoko, Hurrell, John G.R.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Antibodies, Monoclonal - isolation & purification
Cloning, Organism - methods
Hybridomas - immunology
Mice
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Zusammenfassung:Cloning by limiting dilution is a method based on the Poisson distribution. Dilution of cells to an appropriate number per well can maximize the proportion of wells that contain one single clone. Two or more cloning procedures are carried out until >90% of the wells containing single clones are positive for antibody production.
ISSN:1934-3639
1934-3647
DOI:10.1002/0471142727.mb1108s01