HPLC Methods for the Fractionation and Analysis of Negatively Charged Oligosaccharides and Gangliosides

HPLC Methods for the Fractionation and Analysis of Negatively Charged Oligosaccharides and Gangliosides

This unit describes the fractionation and analysis of anionic oligosaccharides and gangliosides using anion‐exchange high‐performance liquid chromatography (HPLC). Saccharides or gangliosides are eluted in order of the number of negative charges they possess, although the charge‐to‐mass ratio can al...

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Veröffentlicht in:Current protocols in molecular biology (Print) 2001-05, Vol.32 (1), p.17.21.1-17.21.8
Hauptverfasser: Manzi, Adriana E., Hayes, Bradley K.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Chromatography, DEAE-Cellulose
Chromatography, High Pressure Liquid - methods
Gangliosides - analysis
Gangliosides - chemistry
Humans
Ions - chemistry
Oligosaccharides - analysis
Oligosaccharides - chemistry
Time Factors
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Beschreibung
Zusammenfassung:This unit describes the fractionation and analysis of anionic oligosaccharides and gangliosides using anion‐exchange high‐performance liquid chromatography (HPLC). Saccharides or gangliosides are eluted in order of the number of negative charges they possess, although the charge‐to‐mass ratio can also contribute to elution position.
ISSN:1934-3639
1934-3647
DOI:10.1002/0471142727.mb1721as32