Identification of putative gene encoded on ORF16 of the 81 kb contig of Arabidopsis thaliana chromosome III as α-mannosidase

Identification of putative gene encoded on ORF16 of the 81 kb contig of Arabidopsis thaliana chromosome III as α-mannosidase

We isolated cDNA corresponding to open reading frame (ORF) 16 of the 81 kb contig of Arabidopsis thaliana chromosome III [Quigley., Nucleic Acids Res., 24, 4313–4318 (1996)] and expressed α-mannosidase activity in tobacco suspension-cultured cells, which revealed that ORF16 encodes α-mannosidase. We...

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Veröffentlicht in:Journal of bioscience and bioengineering 2001, Vol.92 (4), p.401-404
Hauptverfasser: Fujiyama, Kazuhito, Kira, Yuko, Iizuka, Masakazu, Kimura, Yoshinobu, Seki, Tatsuji
Format: Artikel
Sprache:eng
Schlagworte:
ARABIDOPSIS THALIANA
CHROMOSOMES
GENES
genome project
GLYCOSIDASES
isozyme
MANNOSE
α-mannosidase
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Zusammenfassung:We isolated cDNA corresponding to open reading frame (ORF) 16 of the 81 kb contig of Arabidopsis thaliana chromosome III [Quigley., Nucleic Acids Res., 24, 4313–4318 (1996)] and expressed α-mannosidase activity in tobacco suspension-cultured cells, which revealed that ORF16 encodes α-mannosidase. We also suggested that Arabidopsis harbors three genes encoding α-mannosidase by homology search against the database.
ISSN:1389-1723
1347-4421
DOI:10.1016/S1389-1723(01)80248-6