Hepatitis C Virus Nonstructural Protein 5A Inhibits Tumor Necrosis Factor–α–Mediated Apoptosis in Huh7 Cells

To analyze the influence of hepatitis C virus nonstructural protein 5A (NS5A) on apoptosis, we established Huh7 cells that stably express NS5A, and induced apoptosis using tumor necrosis factor (TNF)–α. The viability of control Huh7 cells was reduced to 40%, compared with untreated cells, after TNF-...

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Veröffentlicht in:	The Journal of infectious diseases 2003-11, Vol.188 (10), p.1537-1544
Hauptverfasser:	Miyasaka, Yuka, Enomoto, Nobuyuki, Kurosaki, Masayuki, Sakamoto, Naoya, Kanazawa, Nobuhiko, Kohashi, Takahiro, Ueda, Eri, Maekawa, Shinya, Watanabe, Hideki, Izumi, Namiki, Sato, Chifumi, Watanabe, Mamoru
Format:	Artikel
Sprache:	eng
Schlagworte:	Apoptosis Apoptosis - physiology Arabidopsis Proteins - physiology Biological and medical sciences Caspase 8 Caspase 9 Caspases - physiology Cell Line Chronic hepatitis Dactinomycin - pharmacology Fatty Acid Desaturases - physiology Fundamental and applied biological sciences. Psychology Hepacivirus Hepacivirus - genetics Hepacivirus - metabolism Hepatitis C Hepatitis C - pathology Hepatitis C virus Hepatocytes Hepatocytes - cytology Humans Infections Infectious diseases Medical sciences Microbiology Miscellaneous NF-kappa B - physiology NS5A protein Nucleic Acid Synthesis Inhibitors - pharmacology Oligoribonucleotides, Antisense - genetics Oligoribonucleotides, Antisense - pharmacology Signal Transduction - immunology T lymphocytes Transfection Tumor Necrosis Factor-alpha - antagonists & inhibitors Tumor Necrosis Factor-alpha - physiology Tumor necrosis factors Viability Viral Nonstructural Proteins - genetics Viral Nonstructural Proteins - physiology Virology Viruses
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Zusammenfassung:	To analyze the influence of hepatitis C virus nonstructural protein 5A (NS5A) on apoptosis, we established Huh7 cells that stably express NS5A, and induced apoptosis using tumor necrosis factor (TNF)–α. The viability of control Huh7 cells was reduced to 40%, compared with untreated cells, after TNF-α treatment, whereas that of Huh7-NS5A cells was reduced only to 80%. DNA fragmentation also decreased to
ISSN:	0022-1899 1537-6613
DOI:	10.1086/379253