Effect of lidamycin on the expression of genes involved in invasion regulation in HCT-8 human colon cancer cells

To study the effect of lidamycin on the expression of genes involved in invasion regulation in HCT-8 human colon cancer cells. HCT-8 human colon cancer cells were treated with lidamycin (10 nmol.L-1) for 8 h. The effect of lidamycin on the expression of genes were detected by cDNA arrays. Northern b...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Yao hsüeh hsüeh pao 2001-04, Vol.36 (4), p.246-249
Hauptverfasser:	Cui, D P, Wang, Z, Li, D D
Format:	Artikel
Sprache:	chi
Schlagworte:	Aminoglycosides Anti-Bacterial Agents - pharmacology Antibiotics, Antineoplastic - pharmacology Colonic Neoplasms - genetics Colonic Neoplasms - pathology Enediynes Gene Expression - drug effects Humans Matrix Metalloproteinase 9 - biosynthesis Matrix Metalloproteinase 9 - genetics Neoplasm Invasiveness RNA, Messenger - genetics Tissue Inhibitor of Metalloproteinase-1 - biosynthesis Tissue Inhibitor of Metalloproteinase-1 - genetics Tumor Cells, Cultured
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	To study the effect of lidamycin on the expression of genes involved in invasion regulation in HCT-8 human colon cancer cells. HCT-8 human colon cancer cells were treated with lidamycin (10 nmol.L-1) for 8 h. The effect of lidamycin on the expression of genes were detected by cDNA arrays. Northern blot and RT-PCR. Hybridization of the entire cDNA populations to Atlas Arrays showed that lidamycin down-regulated the expression level of MMP-9 and up-regulated the expression level of TIMP-1. These changes were confirmed by Northern blot and RT-PCR. The results indicate that lidamycin may exhibit its anti-invasive activity by inhibitting the production of type IV collagenase whilst enhancing the production of tissue inhibitor metalloproteinase.
ISSN:	0513-4870