Optimization of the conditions for RAPD-PCR of Candida spp. and Cryptococcus spp

Optimization of the conditions for RAPD-PCR of Candida spp. and Cryptococcus spp

In this study, we determined the optimal RAPD amplification conditions to obtain genetic molecular markers for the rapid and accurate identification of Cryptococcus spp. and Candida spp. The following parameters are modified: template DNA, DNA polymerase, magnesium cloride and primer concentration;...

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Veröffentlicht in:Revista iberoamericana de micología 2001-06, Vol.18 (2), p.65-69
Hauptverfasser: Sansinforiano, M E, Rabasco, A, Martínez-Trancón, M, Parejo, J C, Hermoso-de-Mendoza, M, Padilla, J A
Format: Artikel
Sprache:eng ; spa
Schlagworte:
Candida
Cryptococcus
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Zusammenfassung:In this study, we determined the optimal RAPD amplification conditions to obtain genetic molecular markers for the rapid and accurate identification of Cryptococcus spp. and Candida spp. The following parameters are modified: template DNA, DNA polymerase, magnesium cloride and primer concentration; denaturation, annealing and extension time, temperature of annealing and thermal cycles. After the optimization, reliable and reproducible RAPD patterns are obtained.
ISSN:1130-1406