Mouse embryo fibroblast (MEF) feeder cell preparation
Mitotically inactive mouse embryo fibroblasts (MEFs) are commonly used as feeder layers to prevent the differentiation of mouse embryonic stem (ES) cells. This unit describes the isolation of MEFs and the use of g-irradiation or mitomycin C to produce inactivated feeders suitable for the culture of...
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| Veröffentlicht in: | Current protocols in molecular biology (Print) 2001-05, Vol.Chapter 23 (1), p.Unit 23.2-Unit 23.2 |
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| container_end_page | Unit 23.2 |
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| container_issue | 1 |
| container_start_page | Unit 23.2 |
| container_title | Current protocols in molecular biology (Print) |
| container_volume | Chapter 23 |
| creator | Conner, D A |
| description | Mitotically inactive mouse embryo fibroblasts (MEFs) are commonly used as feeder layers to prevent the differentiation of mouse embryonic stem (ES) cells. This unit describes the isolation of MEFs and the use of g-irradiation or mitomycin C to produce inactivated feeders suitable for the culture of ES cells. |
| doi_str_mv | 10.1002/0471142727.mb2302s51 |
| format | Article |
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This unit describes the isolation of MEFs and the use of g-irradiation or mitomycin C to produce inactivated feeders suitable for the culture of ES cells.</description><subject>Animals</subject><subject>Cell Separation - methods</subject><subject>Dissection - methods</subject><subject>Embryo, Mammalian - cytology</subject><subject>Embryo, Mammalian - physiology</subject><subject>Female</subject><subject>Fibroblasts - cytology</subject><subject>Fibroblasts - physiology</subject><subject>Fibroblasts - radiation effects</subject><subject>Indicators and Reagents</subject><subject>Male</subject><subject>Mice</subject><subject>Mitosis - radiation effects</subject><subject>Pregnancy</subject><issn>1934-3639</issn><issn>1934-3647</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkEtLw0AUhQdRbKn9ByJZiS5S584jM1lKaVVocaPrMI87EEmaOJMu-u9NsbSrexfnOxw-Qu6BLoBS9kKFAhBMMbVoLeOUJQlXZAolFzkvhLo-_7yckHlKP3TECs1BlrdkApoVklE-JXLb7RNm2Np46LJQ29jZxqQhe9qu1s9ZQPQYM4dNk_URexPNUHe7O3ITTJNwfroz8r1efS3f883n28fydZM7poshBy6oxeC1VaiVDGC5ZiCZBwxOgi49MGqkk77wXnEtSu2Md0FyxbkrgM_I439vH7vfPaahaut0HGN2OO6uFHAFiooxKP6DLnYpRQxVH-vWxEMFtDoaqy7GqrOxEXs49e9ti_4CnfzwPwziZVo</recordid><startdate>200105</startdate><enddate>200105</enddate><creator>Conner, D A</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200105</creationdate><title>Mouse embryo fibroblast (MEF) feeder cell preparation</title><author>Conner, D A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c286t-1340befd8b7e875f1b382152d1efc5189d120a5c5d6dd738498cadcf53733c613</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>Cell Separation - methods</topic><topic>Dissection - methods</topic><topic>Embryo, Mammalian - cytology</topic><topic>Embryo, Mammalian - physiology</topic><topic>Female</topic><topic>Fibroblasts - cytology</topic><topic>Fibroblasts - physiology</topic><topic>Fibroblasts - radiation effects</topic><topic>Indicators and Reagents</topic><topic>Male</topic><topic>Mice</topic><topic>Mitosis - radiation effects</topic><topic>Pregnancy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Conner, D A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Current protocols in molecular biology (Print)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Conner, D A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mouse embryo fibroblast (MEF) feeder cell preparation</atitle><jtitle>Current protocols in molecular biology (Print)</jtitle><addtitle>Curr Protoc Mol Biol</addtitle><date>2001-05</date><risdate>2001</risdate><volume>Chapter 23</volume><issue>1</issue><spage>Unit 23.2</spage><epage>Unit 23.2</epage><pages>Unit 23.2-Unit 23.2</pages><issn>1934-3639</issn><eissn>1934-3647</eissn><abstract>Mitotically inactive mouse embryo fibroblasts (MEFs) are commonly used as feeder layers to prevent the differentiation of mouse embryonic stem (ES) cells. This unit describes the isolation of MEFs and the use of g-irradiation or mitomycin C to produce inactivated feeders suitable for the culture of ES cells.</abstract><cop>United States</cop><pmid>18265203</pmid><doi>10.1002/0471142727.mb2302s51</doi></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1934-3639 |
| ispartof | Current protocols in molecular biology (Print), 2001-05, Vol.Chapter 23 (1), p.Unit 23.2-Unit 23.2 |
| issn | 1934-3639 1934-3647 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71371704 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Cell Separation - methods Dissection - methods Embryo, Mammalian - cytology Embryo, Mammalian - physiology Female Fibroblasts - cytology Fibroblasts - physiology Fibroblasts - radiation effects Indicators and Reagents Male Mice Mitosis - radiation effects Pregnancy |
| title | Mouse embryo fibroblast (MEF) feeder cell preparation |
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