Mouse embryo fibroblast (MEF) feeder cell preparation

Mitotically inactive mouse embryo fibroblasts (MEFs) are commonly used as feeder layers to prevent the differentiation of mouse embryonic stem (ES) cells. This unit describes the isolation of MEFs and the use of g-irradiation or mitomycin C to produce inactivated feeders suitable for the culture of...

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Veröffentlicht in:	Current protocols in molecular biology (Print) 2001-05, Vol.Chapter 23 (1), p.Unit 23.2-Unit 23.2
1. Verfasser:	Conner, D A
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cell Separation - methods Dissection - methods Embryo, Mammalian - cytology Embryo, Mammalian - physiology Female Fibroblasts - cytology Fibroblasts - physiology Fibroblasts - radiation effects Indicators and Reagents Male Mice Mitosis - radiation effects Pregnancy
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Beschreibung
Zusammenfassung:	Mitotically inactive mouse embryo fibroblasts (MEFs) are commonly used as feeder layers to prevent the differentiation of mouse embryonic stem (ES) cells. This unit describes the isolation of MEFs and the use of g-irradiation or mitomycin C to produce inactivated feeders suitable for the culture of ES cells.
ISSN:	1934-3639 1934-3647
DOI:	10.1002/0471142727.mb2302s51