Hybridoma Cells Immobilized on Nonwoven Polyester Fabric Discs: Proliferation and Monoclonal Antibody Production in Stationary Culture
Mouse hybridoma cells (O146S) producing a monoclonal antibody against the 146S antigenic particles of O type apthhoviruses were proliferated on the nonwoven polyester fabric (NWPF) discs in a stationary culture. A series of batch experiments were performed in Petri dishes containing RPMI 1640 medium...
Gespeichert in:
| Veröffentlicht in: | Journal of biomaterials applications 2003-10, Vol.18 (2), p.137-148 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 148 |
|---|---|
| container_issue | 2 |
| container_start_page | 137 |
| container_title | Journal of biomaterials applications |
| container_volume | 18 |
| creator | Aslankaraoglu, E Gumusderelioglu, M SI, Gurhan |
| description | Mouse hybridoma cells (O146S) producing a monoclonal antibody against the 146S antigenic particles of O type apthhoviruses were proliferated on the nonwoven polyester fabric (NWPF) discs in a stationary culture. A series of batch experiments were performed in Petri dishes containing RPMI 1640 medium supplemented with 10% (v/v) fetal bovine serum. During the cultivation period glucose and lactate concentrations were measured and monoclonal antibody concentration was determined by sandwich enzyme-linked immunosorbent assay (ELISA). The results showed that in the presence of the NWPF discs cell viability, cell yield, and total monoclonal antibody production was higher than that of the disc-free culture. |
| doi_str_mv | 10.1177/088532803038452 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_71366565</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sage_id>10.1177_088532803038452</sage_id><sourcerecordid>27903752</sourcerecordid><originalsourceid>FETCH-LOGICAL-c418t-b800cbb4a5c9548cc3cd1b4deec0da4e998f63089347b2805eefec1dbb8aa6883</originalsourceid><addsrcrecordid>eNqF0U1PFTEUBuCGaOCCrNmRhkRWjvRzpnVHriIkqCTKetKvISWdFtsZzfUH-Lvt5d4EQ2JcdXGentO3B4AjjN5i3HVnSAhOiUAUUcE42QELzClqBCLkBVisq00tkz2wX8o9QohL1u6CPcxagimVC_D7cqWzt2lUcOlCKPBqHJP2wf9yFqYIP6f4M_1wEd6ksHJlchleqHrDwPe-mPIO3uQU_OCymnzlKlr4KcVkQooqwPM4eZ3saq3sbB6Jj_Dr9KhVXsHlHKY5u1fg5aBCcYfb8wDcXnz4trxsrr98vFqeXzeGYTE1WiBktGaKG8mZMIYaizWzzhlkFXNSiqGlSEjKOl2_hTs3OIOt1kKpVgh6AE43fR9y-j7XPP1YY9TgKro0l77DtG15y_8LSScR7Tip8OQZvE9zruFLj2VHJSaSVXS2QSanUrIb-ofsx5q_x6hfL7J_tsh643jbdtajs09-u7kKXm-BKkaFIatofHlyHNdVk_XoNxtX1J37623_mPsHTBC0Hg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>197391294</pqid></control><display><type>article</type><title>Hybridoma Cells Immobilized on Nonwoven Polyester Fabric Discs: Proliferation and Monoclonal Antibody Production in Stationary Culture</title><source>Access via SAGE</source><source>MEDLINE</source><creator>Aslankaraoglu, E ; Gumusderelioglu, M ; SI, Gurhan</creator><creatorcontrib>Aslankaraoglu, E ; Gumusderelioglu, M ; SI, Gurhan</creatorcontrib><description>Mouse hybridoma cells (O146S) producing a monoclonal antibody against the 146S antigenic particles of O type apthhoviruses were proliferated on the nonwoven polyester fabric (NWPF) discs in a stationary culture. A series of batch experiments were performed in Petri dishes containing RPMI 1640 medium supplemented with 10% (v/v) fetal bovine serum. During the cultivation period glucose and lactate concentrations were measured and monoclonal antibody concentration was determined by sandwich enzyme-linked immunosorbent assay (ELISA). The results showed that in the presence of the NWPF discs cell viability, cell yield, and total monoclonal antibody production was higher than that of the disc-free culture.</description><identifier>ISSN: 0885-3282</identifier><identifier>EISSN: 1530-8022</identifier><identifier>DOI: 10.1177/088532803038452</identifier><identifier>PMID: 14621339</identifier><identifier>CODEN: JBAPEL</identifier><language>eng</language><publisher>Lancaster, PA: SAGE Publications</publisher><subject>Animals ; Antibodies, Monoclonal - biosynthesis ; Biological and medical sciences ; Cell Adhesion - physiology ; Cell Culture Techniques - methods ; Cell Division - physiology ; Cell Line ; Cells, Immobilized - physiology ; Hybridomas - cytology ; Hybridomas - immunology ; Hybridomas - physiology ; Materials Testing ; Medical sciences ; Membranes, Artificial ; Mice ; Polyesters ; Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects) ; Technology. Biomaterials. Equipments. Material. Instrumentation ; Textiles</subject><ispartof>Journal of biomaterials applications, 2003-10, Vol.18 (2), p.137-148</ispartof><rights>2004 INIST-CNRS</rights><rights>Copyright Sage Publications Ltd. Oct 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c418t-b800cbb4a5c9548cc3cd1b4deec0da4e998f63089347b2805eefec1dbb8aa6883</citedby><cites>FETCH-LOGICAL-c418t-b800cbb4a5c9548cc3cd1b4deec0da4e998f63089347b2805eefec1dbb8aa6883</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/088532803038452$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/088532803038452$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,780,784,21819,27924,27925,43621,43622</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15194624$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14621339$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Aslankaraoglu, E</creatorcontrib><creatorcontrib>Gumusderelioglu, M</creatorcontrib><creatorcontrib>SI, Gurhan</creatorcontrib><title>Hybridoma Cells Immobilized on Nonwoven Polyester Fabric Discs: Proliferation and Monoclonal Antibody Production in Stationary Culture</title><title>Journal of biomaterials applications</title><addtitle>J Biomater Appl</addtitle><description>Mouse hybridoma cells (O146S) producing a monoclonal antibody against the 146S antigenic particles of O type apthhoviruses were proliferated on the nonwoven polyester fabric (NWPF) discs in a stationary culture. A series of batch experiments were performed in Petri dishes containing RPMI 1640 medium supplemented with 10% (v/v) fetal bovine serum. During the cultivation period glucose and lactate concentrations were measured and monoclonal antibody concentration was determined by sandwich enzyme-linked immunosorbent assay (ELISA). The results showed that in the presence of the NWPF discs cell viability, cell yield, and total monoclonal antibody production was higher than that of the disc-free culture.</description><subject>Animals</subject><subject>Antibodies, Monoclonal - biosynthesis</subject><subject>Biological and medical sciences</subject><subject>Cell Adhesion - physiology</subject><subject>Cell Culture Techniques - methods</subject><subject>Cell Division - physiology</subject><subject>Cell Line</subject><subject>Cells, Immobilized - physiology</subject><subject>Hybridomas - cytology</subject><subject>Hybridomas - immunology</subject><subject>Hybridomas - physiology</subject><subject>Materials Testing</subject><subject>Medical sciences</subject><subject>Membranes, Artificial</subject><subject>Mice</subject><subject>Polyesters</subject><subject>Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)</subject><subject>Technology. Biomaterials. Equipments. Material. Instrumentation</subject><subject>Textiles</subject><issn>0885-3282</issn><issn>1530-8022</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0U1PFTEUBuCGaOCCrNmRhkRWjvRzpnVHriIkqCTKetKvISWdFtsZzfUH-Lvt5d4EQ2JcdXGentO3B4AjjN5i3HVnSAhOiUAUUcE42QELzClqBCLkBVisq00tkz2wX8o9QohL1u6CPcxagimVC_D7cqWzt2lUcOlCKPBqHJP2wf9yFqYIP6f4M_1wEd6ksHJlchleqHrDwPe-mPIO3uQU_OCymnzlKlr4KcVkQooqwPM4eZ3saq3sbB6Jj_Dr9KhVXsHlHKY5u1fg5aBCcYfb8wDcXnz4trxsrr98vFqeXzeGYTE1WiBktGaKG8mZMIYaizWzzhlkFXNSiqGlSEjKOl2_hTs3OIOt1kKpVgh6AE43fR9y-j7XPP1YY9TgKro0l77DtG15y_8LSScR7Tip8OQZvE9zruFLj2VHJSaSVXS2QSanUrIb-ofsx5q_x6hfL7J_tsh643jbdtajs09-u7kKXm-BKkaFIatofHlyHNdVk_XoNxtX1J37623_mPsHTBC0Hg</recordid><startdate>20031001</startdate><enddate>20031001</enddate><creator>Aslankaraoglu, E</creator><creator>Gumusderelioglu, M</creator><creator>SI, Gurhan</creator><general>SAGE Publications</general><general>Technomic</general><general>Sage Publications Ltd</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7TB</scope><scope>8FD</scope><scope>FR3</scope><scope>JG9</scope><scope>7X8</scope></search><sort><creationdate>20031001</creationdate><title>Hybridoma Cells Immobilized on Nonwoven Polyester Fabric Discs: Proliferation and Monoclonal Antibody Production in Stationary Culture</title><author>Aslankaraoglu, E ; Gumusderelioglu, M ; SI, Gurhan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c418t-b800cbb4a5c9548cc3cd1b4deec0da4e998f63089347b2805eefec1dbb8aa6883</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal - biosynthesis</topic><topic>Biological and medical sciences</topic><topic>Cell Adhesion - physiology</topic><topic>Cell Culture Techniques - methods</topic><topic>Cell Division - physiology</topic><topic>Cell Line</topic><topic>Cells, Immobilized - physiology</topic><topic>Hybridomas - cytology</topic><topic>Hybridomas - immunology</topic><topic>Hybridomas - physiology</topic><topic>Materials Testing</topic><topic>Medical sciences</topic><topic>Membranes, Artificial</topic><topic>Mice</topic><topic>Polyesters</topic><topic>Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects)</topic><topic>Technology. Biomaterials. Equipments. Material. Instrumentation</topic><topic>Textiles</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Aslankaraoglu, E</creatorcontrib><creatorcontrib>Gumusderelioglu, M</creatorcontrib><creatorcontrib>SI, Gurhan</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Materials Research Database</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biomaterials applications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Aslankaraoglu, E</au><au>Gumusderelioglu, M</au><au>SI, Gurhan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hybridoma Cells Immobilized on Nonwoven Polyester Fabric Discs: Proliferation and Monoclonal Antibody Production in Stationary Culture</atitle><jtitle>Journal of biomaterials applications</jtitle><addtitle>J Biomater Appl</addtitle><date>2003-10-01</date><risdate>2003</risdate><volume>18</volume><issue>2</issue><spage>137</spage><epage>148</epage><pages>137-148</pages><issn>0885-3282</issn><eissn>1530-8022</eissn><coden>JBAPEL</coden><abstract>Mouse hybridoma cells (O146S) producing a monoclonal antibody against the 146S antigenic particles of O type apthhoviruses were proliferated on the nonwoven polyester fabric (NWPF) discs in a stationary culture. A series of batch experiments were performed in Petri dishes containing RPMI 1640 medium supplemented with 10% (v/v) fetal bovine serum. During the cultivation period glucose and lactate concentrations were measured and monoclonal antibody concentration was determined by sandwich enzyme-linked immunosorbent assay (ELISA). The results showed that in the presence of the NWPF discs cell viability, cell yield, and total monoclonal antibody production was higher than that of the disc-free culture.</abstract><cop>Lancaster, PA</cop><pub>SAGE Publications</pub><pmid>14621339</pmid><doi>10.1177/088532803038452</doi><tpages>12</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0885-3282 |
| ispartof | Journal of biomaterials applications, 2003-10, Vol.18 (2), p.137-148 |
| issn | 0885-3282 1530-8022 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_71366565 |
| source | Access via SAGE; MEDLINE |
| subjects | Animals Antibodies, Monoclonal - biosynthesis Biological and medical sciences Cell Adhesion - physiology Cell Culture Techniques - methods Cell Division - physiology Cell Line Cells, Immobilized - physiology Hybridomas - cytology Hybridomas - immunology Hybridomas - physiology Materials Testing Medical sciences Membranes, Artificial Mice Polyesters Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects) Technology. Biomaterials. Equipments. Material. Instrumentation Textiles |
| title | Hybridoma Cells Immobilized on Nonwoven Polyester Fabric Discs: Proliferation and Monoclonal Antibody Production in Stationary Culture |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-21T05%3A22%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Hybridoma%20Cells%20Immobilized%20on%20Nonwoven%20Polyester%20Fabric%20Discs:%20Proliferation%20and%20Monoclonal%20Antibody%20Production%20in%20Stationary%20Culture&rft.jtitle=Journal%20of%20biomaterials%20applications&rft.au=Aslankaraoglu,%20E&rft.date=2003-10-01&rft.volume=18&rft.issue=2&rft.spage=137&rft.epage=148&rft.pages=137-148&rft.issn=0885-3282&rft.eissn=1530-8022&rft.coden=JBAPEL&rft_id=info:doi/10.1177/088532803038452&rft_dat=%3Cproquest_cross%3E27903752%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=197391294&rft_id=info:pmid/14621339&rft_sage_id=10.1177_088532803038452&rfr_iscdi=true |