Hybridoma Cells Immobilized on Nonwoven Polyester Fabric Discs: Proliferation and Monoclonal Antibody Production in Stationary Culture

Mouse hybridoma cells (O146S) producing a monoclonal antibody against the 146S antigenic particles of O type apthhoviruses were proliferated on the nonwoven polyester fabric (NWPF) discs in a stationary culture. A series of batch experiments were performed in Petri dishes containing RPMI 1640 medium supplemented with 10% (v/v) fetal bovine serum. During the cultivation period glucose and lactate concentrations were measured and monoclonal antibody concentration was determined by sandwich enzyme-linked immunosorbent assay (ELISA). The results showed that in the presence of the NWPF discs cell viability, cell yield, and total monoclonal antibody production was higher than that of the disc-free culture.