Immobilisation of enzymes on poly(aniline)–poly(anion) composite films. Preparation of bioanodes for biofuel cell applications
Immobilisation of enzymes is important for applications such as biosensors or biofuel cells. A poly(histidine) tag had been introduced on the C terminus of a lactate dehydrogenase enzyme. This mutant enzyme was then immobilised onto poly(aniline) (PANi)–poly(anion) composite films, PANi–poly(vinylsu...
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| Veröffentlicht in: | Bioelectrochemistry (Amsterdam, Netherlands) Netherlands), 2002, Vol.55 (1), p.13-15 |
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| container_title | Bioelectrochemistry (Amsterdam, Netherlands) |
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| creator | Simon, Evelyne Halliwell, Catherine M Toh, Chee Seng Cass, Anthony E.G Bartlett, Philip N |
| description | Immobilisation of enzymes is important for applications such as biosensors or biofuel cells. A poly(histidine) tag had been introduced on the C terminus of a lactate dehydrogenase enzyme. This mutant enzyme was then immobilised onto poly(aniline) (PANi)–poly(anion) composite films, PANi–poly(vinylsulfonate) (PVS) or PANi–poly(acrylate) (PAA). The NADH produced by the immobilised enzyme in the presence of β-nicotinamide adenine dinucleotide (NAD
+) and lactate is oxidised at the poly(aniline)-coated electrode at 0.05 to 0.1 V vs. saturated calomel electrode (SCE) at 35 °C. |
| doi_str_mv | 10.1016/S1567-5394(01)00160-8 |
| format | Article |
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+) and lactate is oxidised at the poly(aniline)-coated electrode at 0.05 to 0.1 V vs. saturated calomel electrode (SCE) at 35 °C.</description><subject>Aniline Compounds</subject><subject>Animals</subject><subject>Anions</subject><subject>Biofuel cell</subject><subject>Electrodes</subject><subject>Enzyme immobilisation</subject><subject>Enzymes, Immobilized - genetics</subject><subject>Enzymes, Immobilized - metabolism</subject><subject>L-Lactate Dehydrogenase - genetics</subject><subject>L-Lactate Dehydrogenase - metabolism</subject><subject>Lactic Acid - chemistry</subject><subject>NAD - biosynthesis</subject><subject>NAD - chemistry</subject><subject>NADH</subject><subject>Poly(aniline) composite films</subject><subject>Polymers</subject><subject>Rabbits</subject><issn>1567-5394</issn><issn>1878-562X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtKxDAUhoMojrdHULoSZ9ExmUzadCUyeANBQQV34TQ5hUjb1KQjjCvfwTf0Scxc1KWrnP_kO_9JfkIOGR0xyrLTByayPBW8mJxQNqSxRVO5QXaYzGUqsvHzZqx_kAHZDeGFUipZLrbJgLFcZpzTHfJx0zSutLUN0FvXJq5KsH2fNxiSqDpXz0-gjdctDr8-Pn-0a4eJdk3ngu0xqWzdhFFy77ED_2tTWgetM9Gocn6hqhnWica6TqDraquXZNgnWxXUAQ_W5x55urx4nF6nt3dXN9Pz21TzjPXpRI4pwCTLq0wXsZCGQllqUQgAoSc6L8xYgimZoLyigBryMSA13JiMiwL5Hjle-Xbevc4w9KqxYfEaaNHNgsoZFzHDIoJiBWrvQvBYqc7bBvxcMaoW0atl9GqRq6JMLaNXMs4drRfMygbN39Q66wicrQCM33yz6FXQFluNxnrUvTLO_rPiGwxrl_M</recordid><startdate>2002</startdate><enddate>2002</enddate><creator>Simon, Evelyne</creator><creator>Halliwell, Catherine M</creator><creator>Toh, Chee Seng</creator><creator>Cass, Anthony E.G</creator><creator>Bartlett, Philip N</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2002</creationdate><title>Immobilisation of enzymes on poly(aniline)–poly(anion) composite films. Preparation of bioanodes for biofuel cell applications</title><author>Simon, Evelyne ; Halliwell, Catherine M ; Toh, Chee Seng ; Cass, Anthony E.G ; Bartlett, Philip N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c361t-4820aa467f6c9aa48d0abbc595aa5c4c79d28adb1503f0aeca72ae0d3dd6359e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Aniline Compounds</topic><topic>Animals</topic><topic>Anions</topic><topic>Biofuel cell</topic><topic>Electrodes</topic><topic>Enzyme immobilisation</topic><topic>Enzymes, Immobilized - genetics</topic><topic>Enzymes, Immobilized - metabolism</topic><topic>L-Lactate Dehydrogenase - genetics</topic><topic>L-Lactate Dehydrogenase - metabolism</topic><topic>Lactic Acid - chemistry</topic><topic>NAD - biosynthesis</topic><topic>NAD - chemistry</topic><topic>NADH</topic><topic>Poly(aniline) composite films</topic><topic>Polymers</topic><topic>Rabbits</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Simon, Evelyne</creatorcontrib><creatorcontrib>Halliwell, Catherine M</creatorcontrib><creatorcontrib>Toh, Chee Seng</creatorcontrib><creatorcontrib>Cass, Anthony E.G</creatorcontrib><creatorcontrib>Bartlett, Philip N</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Bioelectrochemistry (Amsterdam, Netherlands)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Simon, Evelyne</au><au>Halliwell, Catherine M</au><au>Toh, Chee Seng</au><au>Cass, Anthony E.G</au><au>Bartlett, Philip N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immobilisation of enzymes on poly(aniline)–poly(anion) composite films. Preparation of bioanodes for biofuel cell applications</atitle><jtitle>Bioelectrochemistry (Amsterdam, Netherlands)</jtitle><addtitle>Bioelectrochemistry</addtitle><date>2002</date><risdate>2002</risdate><volume>55</volume><issue>1</issue><spage>13</spage><epage>15</epage><pages>13-15</pages><issn>1567-5394</issn><eissn>1878-562X</eissn><abstract>Immobilisation of enzymes is important for applications such as biosensors or biofuel cells. A poly(histidine) tag had been introduced on the C terminus of a lactate dehydrogenase enzyme. This mutant enzyme was then immobilised onto poly(aniline) (PANi)–poly(anion) composite films, PANi–poly(vinylsulfonate) (PVS) or PANi–poly(acrylate) (PAA). The NADH produced by the immobilised enzyme in the presence of β-nicotinamide adenine dinucleotide (NAD
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Aniline Compounds Animals Anions Biofuel cell Electrodes Enzyme immobilisation Enzymes, Immobilized - genetics Enzymes, Immobilized - metabolism L-Lactate Dehydrogenase - genetics L-Lactate Dehydrogenase - metabolism Lactic Acid - chemistry NAD - biosynthesis NAD - chemistry NADH Poly(aniline) composite films Polymers Rabbits |
| title | Immobilisation of enzymes on poly(aniline)–poly(anion) composite films. Preparation of bioanodes for biofuel cell applications |
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