Immobilisation of enzymes on poly(aniline)–poly(anion) composite films. Preparation of bioanodes for biofuel cell applications

Immobilisation of enzymes on poly(aniline)–poly(anion) composite films. Preparation of bioanodes for biofuel cell applications

Immobilisation of enzymes is important for applications such as biosensors or biofuel cells. A poly(histidine) tag had been introduced on the C terminus of a lactate dehydrogenase enzyme. This mutant enzyme was then immobilised onto poly(aniline) (PANi)–poly(anion) composite films, PANi–poly(vinylsu...

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Veröffentlicht in:Bioelectrochemistry (Amsterdam, Netherlands) Netherlands), 2002, Vol.55 (1), p.13-15
Hauptverfasser: Simon, Evelyne, Halliwell, Catherine M, Toh, Chee Seng, Cass, Anthony E.G, Bartlett, Philip N
Format: Artikel
Sprache:eng
Schlagworte:
Aniline Compounds
Animals
Anions
Biofuel cell
Electrodes
Enzyme immobilisation
Enzymes, Immobilized - genetics
Enzymes, Immobilized - metabolism
L-Lactate Dehydrogenase - genetics
L-Lactate Dehydrogenase - metabolism
Lactic Acid - chemistry
NAD - biosynthesis
NAD - chemistry
NADH
Poly(aniline) composite films
Polymers
Rabbits
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Zusammenfassung:Immobilisation of enzymes is important for applications such as biosensors or biofuel cells. A poly(histidine) tag had been introduced on the C terminus of a lactate dehydrogenase enzyme. This mutant enzyme was then immobilised onto poly(aniline) (PANi)–poly(anion) composite films, PANi–poly(vinylsulfonate) (PVS) or PANi–poly(acrylate) (PAA). The NADH produced by the immobilised enzyme in the presence of β-nicotinamide adenine dinucleotide (NAD +) and lactate is oxidised at the poly(aniline)-coated electrode at 0.05 to 0.1 V vs. saturated calomel electrode (SCE) at 35 °C.
ISSN:1567-5394
1878-562X
DOI:10.1016/S1567-5394(01)00160-8