pHP489, a Helicobacter pylori small cryptic plasmid, harbors a novel gene coding for a replication initiation protein

We have analyzed a Helicobacter pylori plasmid, pHP489. The 1222-bp nucleotide sequence had one open reading frame, a DnaA-binding site, one direct repeat, and three inverted repeats. The (G + C) content of pHP489 was 33.3%. Although the nucleic acid sequence and deduced amino acid sequence were hom...

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Veröffentlicht in:Plasmid 2003-11, Vol.50 (3), p.236-241
Hauptverfasser: Song, Jae-Young, Park, Seong-Gyu, Kang, Hyung-Lyun, Lee, Woo-Kon, Cho, Myung-Je, Park, Jeong-Uck, Baik, Seung-Chul, Youn, Hee-Shang, Ko, Gyung-Hyuck, Rhee, Kwang-Ho
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Sprache:eng
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Zusammenfassung:We have analyzed a Helicobacter pylori plasmid, pHP489. The 1222-bp nucleotide sequence had one open reading frame, a DnaA-binding site, one direct repeat, and three inverted repeats. The (G + C) content of pHP489 was 33.3%. Although the nucleic acid sequence and deduced amino acid sequence were homologous to those of other bacterial plasmid Rep proteins, the degree of similarity was very low. A deletion analysis showed that the Rep protein was not required for the replication of pHP489 in its H. pylori host, but the host replication machinery was needed.
ISSN:0147-619X
1095-9890
DOI:10.1016/S0147-619X(03)00061-1