pHP489, a Helicobacter pylori small cryptic plasmid, harbors a novel gene coding for a replication initiation protein
We have analyzed a Helicobacter pylori plasmid, pHP489. The 1222-bp nucleotide sequence had one open reading frame, a DnaA-binding site, one direct repeat, and three inverted repeats. The (G + C) content of pHP489 was 33.3%. Although the nucleic acid sequence and deduced amino acid sequence were hom...
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Veröffentlicht in: | Plasmid 2003-11, Vol.50 (3), p.236-241 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | We have analyzed a
Helicobacter pylori plasmid, pHP489. The 1222-bp nucleotide sequence had one open reading frame, a DnaA-binding site, one direct repeat, and three inverted repeats. The (G
+
C) content of pHP489 was 33.3%. Although the nucleic acid sequence and deduced amino acid sequence were homologous to those of other bacterial plasmid Rep proteins, the degree of similarity was very low. A deletion analysis showed that the Rep protein was not required for the replication of pHP489 in its
H. pylori host, but the host replication machinery was needed. |
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ISSN: | 0147-619X 1095-9890 |
DOI: | 10.1016/S0147-619X(03)00061-1 |