Preparation of poly(thionine) modified screen-printed carbon electrode and its application to determine NADH in flow injection analysis system

A poly(thionine) modified screen-printed carbon electrode has been prepared by an electrooxidative polymerization of thionine in neutral phosphate buffer. The modified electrodes are found to give stable and reproducible electrocatlytic responses to NADH and exhibit good stability. Several technique...

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Veröffentlicht in:	Biosensors & bioelectronics 2003-11, Vol.19 (3), p.277-282
Hauptverfasser:	Gao, Qiang, Cui, Xiaoqiang, Yang, Fan, Ma, Ying, Yang, Xiurong
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biosensing Techniques - instrumentation Biosensing Techniques - methods Biosensors Biotechnology Carbon - chemistry Coated Materials, Biocompatible - chemical synthesis Coated Materials, Biocompatible - chemistry Electrochemistry - instrumentation Electrochemistry - methods Electrodes Electropolymerization Equipment Design Equipment Failure Analysis FIA Flow Injection Analysis - instrumentation Flow Injection Analysis - methods Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies NAD - analysis NAD - chemistry NADH Phenothiazines - chemical synthesis Phenothiazines - chemistry Polymers - chemical synthesis Reproducibility of Results Screen-printed carbon electrode Sensitivity and Specificity Surface Properties Thionine Various methods and equipments
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Zusammenfassung:	A poly(thionine) modified screen-printed carbon electrode has been prepared by an electrooxidative polymerization of thionine in neutral phosphate buffer. The modified electrodes are found to give stable and reproducible electrocatlytic responses to NADH and exhibit good stability. Several techniques, including cyclic voltammetry, X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM), have been employed to characterize the poly(thionine) film. Further, the modified screen-printed carbon electrode was found to be promising as an amperometric detector for the flow injection analysis (FIA) of NADH, typically with a dynamic range of 5–100 μM.
ISSN:	0956-5663 1873-4235
DOI:	10.1016/S0956-5663(03)00212-4